Aerial Ammonia Exposure Induces the Perturbation of the Interorgan Ammonia Disposal and Branched-chain Amino Acid Catabolism in Growing Pigs

Overview

Journal Anim Nutr

Date 2021 Oct 27

PMID 34703912

Authors

Shanlong Tang

Chang Yin

Jingjing Xie

Jinglin Jiao

Liang Chen

Lei Liu

Sheng Zhang

Hongfu Zhang

Affiliations

Soon will be listed here.

Abstract

Aerial ammonia exposure leads to tissue damage and metabolic dysfunction. However, it is unclear how different organs are coordinated to defend against aerial ammonia exposure. Twenty-four pigs were randomly divided into 4 groups, exposed to 0, 10, 25 or 35 mg/m ammonia respectively for 25 d. After above 25 mg/m ammonia exposure, decreased aspartate ( = 0.016), glutamate ( = 0.030) and increased ornithine ( = 0.002) were found in the ammonia-removing liver, and after high ammonia (35 mg/m) exposure, glutamine synthetase () expression was increased ( = 0.012). An increased glutamate ( = 0.004) and decreased glutaminase () expression ( = 0.083) were observed in the lungs after high ammonia exposure. There was also an increasing trend of glutamine in the kidneys after high ammonia exposure ( = 0.066). For branched-chain amino acid (BCAA) catabolism, high ammonia exposure increased BCAA content in both the lungs and muscle ( < 0.05), whereas below 25 mg/m ammonia exposure increased BCAA only in the lungs ( < 0.05). The expression of BCAA transaminase () and dehydrogenase complex ( and ) were inhibited to a varying degree in the liver, lungs and muscle after above 25 mg/m ammonia exposure, especially high ammonia exposure. The expression of complex and glutamate-glutamine metabolism-related genes were highly expressed in the liver, followed by the lungs and muscle ( < 0.01), whereas the expression was highest in the lungs ( = 0.002). Altogether, low ammonia exposure sufficed to evoke the urea cycle to detoxify ammonia in the liver. The process of ammonia removal in the liver and potential ability of the lungs to detoxify ammonia were enhanced with increasing ammonia. Furthermore, high ammonia exposure impaired the BCAA catabolism and decreased the transcripts of the BCAA catabolism-related enzymes, resulting in high BCAA content in extrahepatic tissues. Therefore, with aerial ammonia increasing, an increased urea cycle and glutamine synthesis were ammonia defensive strategies, and high ammonia exposure impaired the BCAA catabolism.

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