Coculture Method for Cultivation of Uncultured Oral Bacteria

Overview

Journal J Oral Maxillofac Pathol

Date 2021 Oct 27

PMID 34703120

Citations 1

Authors

Preethi Kusugal

Kishore G Bhat

Preeti Ingalagi

Sanjivani Patil

Geeta Pattar

Affiliations

Soon will be listed here.

Abstract

Purpose: The purpose of the study is to culture uncultured oral bacteria with helper strains using the coculture method from the subgingival plaque samples of chronic periodontitis patients.

Materials And Methods: The samples were processed and inoculated on a blood agar medium enriched with hemin and Vitamin K. A helper strain (ATCC 6919) was cross-streaked across the inoculums to facilitate coculture. The plates were then incubated for 7 days with subsequent subculturing and further incubation.

Results: Satellite colonies around helper strain showed one colony type of Porphyromonas gingivalis, one was of nonpigmented , three were of and five isolates remained unidentified.

Conclusions: Coculture could be used effectively as one of the methods in the isolation and cultivation of oral bacteria. Incubation using the anaerobic jar technique was found to be economical and efficient for the growth of anaerobic oral bacteria.

Citing Articles

Portable biosensor-based oral pathogenic bacteria detection for community and family applications.

Ming J, Zeng X, Zhou R Anal Bioanal Chem. 2023; 415(21):5221-5233.

PMID: 37389598 DOI: 10.1007/s00216-023-04809-1.

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