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Establishment and Characterisation of Metastatic Extraskeletal Ewing Sarcoma Mouse Models

Overview
Journal In Vivo
Specialty Oncology
Date 2021 Oct 26
PMID 34697140
Citations 3
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Abstract

Background/aim: Ewing sarcomas most commonly arise in the bones, but can also manifest as extraskeletal tumours in soft tissues. Metastases from extraskeletal Ewing sarcomas occur in more diverse anatomical sites than skeletal tumours, and have poorer survival rates. Few animal models replicate the extraskeletal form of Ewing sarcoma, and those that have been developed do not reflect the widespread metastatic spread of these cancers.

Materials And Methods: Luciferase-expressing Ewing sarcoma cells derived from a muscle tumour were intramuscularly or intravenously injected into nude mice.

Results: Both models achieved metastatic spread to numerous sites including the lungs, liver, kidneys, and brain. We characterized the cellular composition of primary and metastatic tumours, observing a greater level of immune cell infiltration in metastases compared to primary intramuscular tumours.

Conclusion: These pre-clinical models will hopefully facilitate the evaluation of novel therapies and contribute to better understanding the disease progression of metastatic extraskeletal Ewing sarcoma.

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References
1.
Roberts I, Wienberg J, Nacheva E, Grace C, Griffin D, Coleman N . Novel method for the production of multiple colour chromosome paints for use in karyotyping by fluorescence in situ hybridisation. Genes Chromosomes Cancer. 1999; 25(3):241-50. DOI: 10.1002/(sici)1098-2264(199907)25:3<241::aid-gcc6>3.0.co;2-7. View

2.
Applebaum M, Worch J, Matthay K, Goldsby R, Neuhaus J, West D . Clinical features and outcomes in patients with extraskeletal Ewing sarcoma. Cancer. 2011; 117(13):3027-32. PMC: 3135782. DOI: 10.1002/cncr.25840. View

3.
Yu M, Selvaraj S, Liang-Chu M, Aghajani S, Busse M, Yuan J . A resource for cell line authentication, annotation and quality control. Nature. 2015; 520(7547):307-11. DOI: 10.1038/nature14397. View

4.
Lopez-Alemany R, Tirado O . Metastasis Assessment in Ewing Sarcoma Using Orthotopic Xenografts. Methods Mol Biol. 2020; 2226:201-213. DOI: 10.1007/978-1-0716-1020-6_16. View

5.
Womer R, West D, Krailo M, Dickman P, Pawel B, Grier H . Randomized controlled trial of interval-compressed chemotherapy for the treatment of localized Ewing sarcoma: a report from the Children's Oncology Group. J Clin Oncol. 2012; 30(33):4148-54. PMC: 3494838. DOI: 10.1200/JCO.2011.41.5703. View