From Angstroms to Nanometers: Measuring Interatomic Distances by Solid-State NMR

Overview

Journal Chem Rev

Publisher American Chemical Society

Specialty Chemistry

Date 2021 Oct 25

PMID 34694769

Citations 16

Authors

Alexander A Shcherbakov

Joao Medeiros-Silva

Nhi Tran

Martin D Gelenter

Mei Hong

Affiliations

Soon will be listed here.

Abstract

Internuclear distances represent one of the main structural constraints in molecular structure determination using solid-state NMR spectroscopy, complementing chemical shifts and orientational restraints. Although a large number of magic-angle-spinning (MAS) NMR techniques have been available for distance measurements, traditional C and N NMR experiments are inherently limited to distances of a few angstroms due to the low gyromagnetic ratios of these nuclei. Recent development of fast MAS triple-resonance F and H NMR probes has stimulated the design of MAS NMR experiments that measure distances in the 1-2 nm range with high sensitivity. This review describes the principles and applications of these multiplexed multidimensional correlation distance NMR experiments, with an emphasis on F- and H-based distance experiments. Representative applications of these long-distance NMR methods to biological macromolecules as well as small molecules are reviewed.

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