CWI Pathway Participated in Vegetative Growth and Pathogenicity Through a Downstream Effector AflRlm1 in

Overview

Journal iScience

Publisher Cell Press

Date 2021 Oct 25

PMID 34693219

Citations 7

Authors

Can Tan

Ji-Li Deng

Feng Zhang

Zhuo Zhu

Li-Juan Yan

Meng-Juan Zhang

Jun Yuan

Shi-Hua Wang

Affiliations

Soon will be listed here.

Abstract

The cell wall is an essential dynamic structure for shielding fungus from environmental stress, and its synthesizing and remodeling are regulated by the cell wall integrity (CWI) pathway. Here, we explored the roles of a putative downstream effector AflRlm1 of CWI pathway in . The results showed that AflRlm1 played a positive role in conidia production, sclerotium formation, aflatoxin biosynthesis, and pathogenicity. Furthermore, we provided evidence for the physical connection between AflRlm1 and AflSlt2 and determined the role of AflSlt2 in the phosphorylation of AflRlm1. Then, we discovered the importance of WSCs (cell wall integrity and stress response component) to the CWI signal and the process of AflRlm1 transferring to the nucleus after receiving the signal. Overall, this study clarified the transmission process of CWI signals and proves that the CWI pathway plays a key role in the development of and the production of aflatoxin combined with transcriptome data analysis.

Citing Articles

S-palmitoylation of MAP kinase is essential for fungal virulence.

Duan Y, Li P, Zhang D, Wang L, Fang Y, Hu H mBio. 2024; 15(12):e0270424.

PMID: 39470248 PMC: 11633104. DOI: 10.1128/mbio.02704-24.

The target of rapamycin signaling pathway regulates vegetative development, aflatoxin biosynthesis, and pathogenicity in .

Li G, Cao X, Tumukunde E, Zeng Q, Wang S Elife. 2024; 12.

PMID: 38990939 PMC: 11239180. DOI: 10.7554/eLife.89478.

Comparative Transcriptomic Analyses Propose the Molecular Regulatory Mechanisms Underlying 1,8-Cineole from Hay and Promote the Asexual Sporulation of in Submerged Fermentation.

Li H, Dai J, Wang J, Lu C, Luo Z, Zheng X Molecules. 2023; 28(22).

PMID: 38005233 PMC: 10672923. DOI: 10.3390/molecules28227511.

The Transcription Factor CcRlm1 Regulates Cell Wall Maintenance and Poplar Defense Response by Directly Targeting and in Cytospora chrysosperma.

Xie D, Tang C, Wang Y, Jin H, Wang Y Appl Environ Microbiol. 2023; 89(6):e0066123.

PMID: 37289076 PMC: 10304682. DOI: 10.1128/aem.00661-23.

Genomic and Phenotypic Trait Variation of the Opportunistic Human Pathogen Aspergillus flavus and Its Close Relatives.

Hatmaker E, Rangel-Grimaldo M, Raja H, Pourhadi H, Knowles S, Fuller K Microbiol Spectr. 2022; 10(6):e0306922.

PMID: 36318036 PMC: 9769809. DOI: 10.1128/spectrum.03069-22.

References

Jung U, Sobering A, Romeo M, Levin D . Regulation of the yeast Rlm1 transcription factor by the Mpk1 cell wall integrity MAP kinase. Mol Microbiol. 2002; 46(3):781-9. DOI: 10.1046/j.1365-2958.2002.03198.x. View

Mattos E, Silva L, Valero C, de Castro P, Dos Reis T, Ribeiro L . The Aspergillus fumigatus Phosphoproteome Reveals Roles of High-Osmolarity Glycerol Mitogen-Activated Protein Kinases in Promoting Cell Wall Damage and Caspofungin Tolerance. mBio. 2020; 11(1). PMC: 7002344. DOI: 10.1128/mBio.02962-19. View

Oliveira-Pacheco J, Alves R, Costa-Barbosa A, Cerqueira-Rodrigues B, Pereira-Silva P, Paiva S . The Role of Transcription Factor in Response to Carbon Adaptation. Front Microbiol. 2018; 9:1127. PMC: 5986929. DOI: 10.3389/fmicb.2018.01127. View

Heredia M, Ikeh M, Gunasekaran D, Conrad K, Filimonava S, Marotta D . An expanded cell wall damage signaling network is comprised of the transcription factors Rlm1 and Sko1 in Candida albicans. PLoS Genet. 2020; 16(7):e1008908. PMC: 7371209. DOI: 10.1371/journal.pgen.1008908. View

Verna J, Lodder A, Lee K, Vagts A, Ballester R . A family of genes required for maintenance of cell wall integrity and for the stress response in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1998; 94(25):13804-9. PMC: 28388. DOI: 10.1073/pnas.94.25.13804. View

Wilk S, Wittland J, Thywissen A, Schmitz H, Heinisch J . A block of endocytosis of the yeast cell wall integrity sensors Wsc1 and Wsc2 results in reduced fitness in vivo. Mol Genet Genomics. 2010; 284(3):217-29. DOI: 10.1007/s00438-010-0563-2. View

Weaver M, Mack B, Gilbert M . Genome Sequences of 20 Georeferenced Aspergillus flavus Isolates. Microbiol Resour Announc. 2019; 8(11). PMC: 6424216. DOI: 10.1128/MRA.01718-18. View

Chang P, Ehrlich K, Hua S . Cladal relatedness among Aspergillus oryzae isolates and Aspergillus flavus S and L morphotype isolates. Int J Food Microbiol. 2006; 108(2):172-7. DOI: 10.1016/j.ijfoodmicro.2005.11.008. View

Heinemann S, Symoens F, Gordts B, Jannes H, Nolard N . Environmental investigations and molecular typing of Aspergillus flavus during an outbreak of postoperative infections. J Hosp Infect. 2004; 57(2):149-55. DOI: 10.1016/j.jhin.2004.02.007. View

10.

Tumukunde E, Li D, Qin L, Li Y, Shen J, Wang S . Osmotic-Adaptation Response of sakA/hogA Gene to Aflatoxin Biosynthesis, Morphology Development and Pathogenicity in Aspergillus flavus. Toxins (Basel). 2019; 11(1). PMC: 6356625. DOI: 10.3390/toxins11010041. View

11.

Tong S, Chen Y, Zhu J, Ying S, Feng M . Subcellular localization of five singular WSC domain-containing proteins and their roles in Beauveria bassiana responses to stress cues and metal ions. Environ Microbiol Rep. 2016; 8(2):295-304. DOI: 10.1111/1758-2229.12380. View

12.

Cruz S, Munoz S, Manjon E, Garcia P, Sanchez Y . The fission yeast cell wall stress sensor-like proteins Mtl2 and Wsc1 act by turning on the GTPase Rho1p but act independently of the cell wall integrity pathway. Microbiologyopen. 2013; 2(5):778-94. PMC: 3831639. DOI: 10.1002/mbo3.113. View

13.

Maddi A, Dettman A, Fu C, Seiler S, Free S . WSC-1 and HAM-7 are MAK-1 MAP kinase pathway sensors required for cell wall integrity and hyphal fusion in Neurospora crassa. PLoS One. 2012; 7(8):e42374. PMC: 3411791. DOI: 10.1371/journal.pone.0042374. View

14.

He Z, Song Y, Deng J, Zhao X, Qin X, Luo Z . Participation of a MADS-box transcription factor, Mb1, in regulation of the biocontrol potential in an insect fungal pathogen. J Invertebr Pathol. 2020; 170:107335. DOI: 10.1016/j.jip.2020.107335. View

15.

Yang G, Hu Y, Fasoyin O, Yue Y, Chen L, Qiu Y . The Phosphatase CDC14 Regulates Development, Aflatoxin Biosynthesis and Pathogenicity. Front Cell Infect Microbiol. 2018; 8:141. PMC: 5950752. DOI: 10.3389/fcimb.2018.00141. View

16.

Nishida N, Jing D, Kuroda K, Ueda M . Activation of signaling pathways related to cell wall integrity and multidrug resistance by organic solvent in Saccharomyces cerevisiae. Curr Genet. 2014; 60(3):149-62. DOI: 10.1007/s00294-013-0419-5. View

17.

Zeng F, Gong X, Hamid M, Fu Y, Jiatao X, Cheng J . A fungal cell wall integrity-associated MAP kinase cascade in Coniothyrium minitans is required for conidiation and mycoparasitism. Fungal Genet Biol. 2012; 49(5):347-57. DOI: 10.1016/j.fgb.2012.02.008. View

18.

Fuchs B, Mylonakis E . Our paths might cross: the role of the fungal cell wall integrity pathway in stress response and cross talk with other stress response pathways. Eukaryot Cell. 2009; 8(11):1616-25. PMC: 2772411. DOI: 10.1128/EC.00193-09. View

19.

Miyazaki T, Inamine T, Yamauchi S, Nagayoshi Y, Saijo T, Izumikawa K . Role of the Slt2 mitogen-activated protein kinase pathway in cell wall integrity and virulence in Candida glabrata. FEMS Yeast Res. 2010; 10(3):343-52. DOI: 10.1111/j.1567-1364.2010.00611.x. View

20.

Zhang F, Xu G, Geng L, Lu X, Yang K, Yuan J . The Stress Response Regulator AflSkn7 Influences Morphological Development, Stress Response, and Pathogenicity in the Fungus Aspergillus flavus. Toxins (Basel). 2016; 8(7). PMC: 4963835. DOI: 10.3390/toxins8070202. View