Genome-Wide Identification and Comparative Analysis of the Teosinte Branched 1/Cycloidea/Proliferating Cell Factors 1/2 Transcription Factors Related to Anti-cancer Drug Camptothecin Biosynthesis in

Overview

Journal Front Plant Sci

Date 2021 Oct 25

PMID 34691124

Citations 3

Authors

Can Wang

Xiaolong Hao

Yao Wang

Min Shi

Zhi-Gang Zhou

Guoyin Kai

Affiliations

Soon will be listed here.

Abstract

(; ) is a medicinal herbaceous plant, which can accumulate camptothecin (CPT). CPT and its derivatives are widely used as chemotherapeutic drugs for treating malignant tumors. Its biosynthesis pathway has been attracted significant attention. Teosinte branched 1/cycloidea/proliferating cell factors 1/2 (TCP) transcription factors (TFs) regulate a variety of physiological processes, while TCP TFs are involved in the regulation of CPT biosynthesis remain unclear. In this study, a systematic analysis of the TCP TFs family in was performed. A total of 16 TCP () genes were identified and categorized into two subgroups based on their phylogenetic relationships with those in . Tissue-specific expression patterns revealed that nine genes showed the highest expression levels in leaves, while the other seven showed a higher expression level in the stems. Co-expression, phylogeny analysis, and dual-luciferase (Dual-LUC) assay revealed that potentially plays important role in CPT and its precursor biosynthesis. In addition, the subcellular localization experiment of candidate genes showed that they are all localized in the nucleus. Our study lays a foundation for further functional characterization of the candidate genes involved in CPT biosynthesis regulation and provides new strategies for increasing CPT production.

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