Expression of MiR-31 in Saliva-liquid Biopsy in Patients with Oral Squamous Cell Carcinoma

Overview

Journal J Taibah Univ Med Sci

Specialty General Medicine

Date 2021 Oct 25

PMID 34690655

Citations 8

Authors

Parma Kumari

Sofia A Syed

Mohsin Wahid

Muhammad A Qureshi

Rajesh Kumar

Affiliations

Soon will be listed here.

Abstract

Objectives: Oral squamous cell carcinoma (OSCC) is a commonly reported cancer in men and is second only to breast cancer in women in Pakistan.. Investigations for identifying biomarkers of OSCC are essential for diagnostic, therapeutic, or prognostic significance. This study aims to examine the miR-31 expression in the pre- and post-operative OSCC patients and correlate this expression with clinicopathological characteristics.

Methods: Patients with histopathologically confirmed OSCC who had undergone surgical resections of tumours were recruited. A total of 40 saliva samples (pre- and post-operative) were collected from 19 patients and two healthy individuals. Levels of salivary miR-31 expressions were examined through quantitative reverse transcription polymerase chain reaction.

Results: The salivary miR-31 expression was significantly higher in the preoperative patients than in postoperative patients ( < 0.001). However, no significant correlation had been found between the salivary miR-31 expression and clinicopathological characteristics ( > 0.05).

Conclusion: Our data suggest that miR-31 can be used as an adjunct non-invasive marker to monitor surgery outcomes during postoperative follow-up in patients with OSCC.

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References

Eguchi T . Organoids and Liquid Biopsy in Oral Cancer Research. J Clin Med. 2020; 9(11). PMC: 7698863. DOI: 10.3390/jcm9113701. View

Uma Maheswari T, Nivedhitha M, Ramani P . Expression profile of salivary micro RNA-21 and 31 in oral potentially malignant disorders. Braz Oral Res. 2020; 34:e002. DOI: 10.1590/1807-3107bor-2020.vol34.0002. View

Bhat M, Advani J, Rajagopalan P, Patel K, Nanjappa V, Solanki H . Cigarette smoke and chewing tobacco alter expression of different sets of miRNAs in oral keratinocytes. Sci Rep. 2018; 8(1):7040. PMC: 5935709. DOI: 10.1038/s41598-018-25498-2. View

Severino P, Oliveira L, Andreghetto F, Torres N, Curioni O, Cury P . Small RNAs in metastatic and non-metastatic oral squamous cell carcinoma. BMC Med Genomics. 2015; 8:31. PMC: 4479233. DOI: 10.1186/s12920-015-0102-4. View

Lu Z, He Q, Liang J, Li W, Su Q, Chen Z . miR-31-5p Is a Potential Circulating Biomarker and Therapeutic Target for Oral Cancer. Mol Ther Nucleic Acids. 2019; 16:471-480. PMC: 6495075. DOI: 10.1016/j.omtn.2019.03.012. View

Qureshi M, Khan S, Sharafat S, Quraishy M . Common Cancers in Karachi, Pakistan: 2010-2019 Cancer Data from the Dow Cancer Registry. Pak J Med Sci. 2020; 36(7):1572-1578. PMC: 7674861. DOI: 10.12669/pjms.36.7.3056. View

Fang C, Li Y . Prospective applications of microRNAs in oral cancer. Oncol Lett. 2019; 18(4):3974-3984. PMC: 6757290. DOI: 10.3892/ol.2019.10751. View

Ali J, Sabiha B, Jan H, Haider S, Khan A, Ali S . Genetic etiology of oral cancer. Oral Oncol. 2017; 70:23-28. DOI: 10.1016/j.oraloncology.2017.05.004. View

Yu T, Ma P, Wu D, Shu Y, Gao W . Functions and mechanisms of microRNA-31 in human cancers. Biomed Pharmacother. 2018; 108:1162-1169. DOI: 10.1016/j.biopha.2018.09.132. View

10.

Liu C, Kao S, Tu H, Tsai M, Chang K, Lin S . Increase of microRNA miR-31 level in plasma could be a potential marker of oral cancer. Oral Dis. 2010; 16(4):360-4. DOI: 10.1111/j.1601-0825.2009.01646.x. View

11.

Ouyang S, Wang J, Huang Z, Liao L . [Expression of microRNA-31 and its clinicopathologic significance in oral squamous cell carcinoma]. Zhonghua Kou Qiang Yi Xue Za Zhi. 2013; 48(8):481-4. View

12.

Weber J, Baxter D, Zhang S, Huang D, Huang K, Lee M . The microRNA spectrum in 12 body fluids. Clin Chem. 2010; 56(11):1733-41. PMC: 4846276. DOI: 10.1373/clinchem.2010.147405. View

13.

Bottani M, Banfi G, Lombardi G . Circulating miRNAs as Diagnostic and Prognostic Biomarkers in Common Solid Tumors: Focus on Lung, Breast, Prostate Cancers, and Osteosarcoma. J Clin Med. 2019; 8(10). PMC: 6833074. DOI: 10.3390/jcm8101661. View

14.

Siow M, Ng L, Vincent-Chong V, Jamaludin M, Abraham M, Abdul Rahman Z . Dysregulation of miR-31 and miR-375 expression is associated with clinical outcomes in oral carcinoma. Oral Dis. 2013; 20(4):345-51. DOI: 10.1111/odi.12118. View

15.

Basharat S, Shaikh B, Rashid H, Rashid M . Health seeking behaviour, delayed presentation and its impact among oral cancer patients in Pakistan: a retrospective qualitative study. BMC Health Serv Res. 2019; 19(1):715. PMC: 6805330. DOI: 10.1186/s12913-019-4521-3. View

16.

Hung K, Liu C, Chiu P, Lin J, Chang K, Shih W . MicroRNA-31 upregulation predicts increased risk of progression of oral potentially malignant disorder. Oral Oncol. 2015; 53:42-7. DOI: 10.1016/j.oraloncology.2015.11.017. View

17.

Ahmed R, Malik S, Khan M, Khattak M . Epidemiological and clinical correlates of oral squamous cell carcinoma in patients from north-west Pakistan. J Pak Med Assoc. 2019; 69(8):1074-1078. View

18.

Wang L, Li H, Yang Y, Su Y, Lian J, Li T . MiR-31 is a potential biomarker for diagnosis of head and neck squamous cell carcinoma. Int J Clin Exp Pathol. 2020; 11(9):4339-4345. PMC: 6962945. View

19.

Peng Q, Cheng Y, Zhang W, Fan H, Mao Q, Xu P . circRNA_0000140 suppresses oral squamous cell carcinoma growth and metastasis by targeting miR-31 to inhibit Hippo signaling pathway. Cell Death Dis. 2020; 11(2):112. PMC: 7010827. DOI: 10.1038/s41419-020-2273-y. View

20.

Liu C, Lin S, Yang C, Cheng H, Chang K . Exploiting salivary miR-31 as a clinical biomarker of oral squamous cell carcinoma. Head Neck. 2011; 34(2):219-24. DOI: 10.1002/hed.21713. View