Genetic Mapping of the Locus Causing Hybrid Lethality in Interspecific Hybrids

Overview

Journal Plants (Basel)

Date 2021 Oct 23

PMID 34685871

Citations 5

Authors

Takahiro Tezuka

Naoto Kitamura

Sae Imagawa

Akira Hasegawa

Kumpei Shiragaki

Hai He

Masanori Yanase

Yoshiyuki Ogata

Toshinobu Morikawa

Shuji Yokoi

Affiliations

Soon will be listed here.

Abstract

Hybrid lethality, a postzygotic mechanism of reproductive isolation, is a phenomenon that causes the death of F hybrid seedlings. Hybrid lethality is generally caused by the epistatic interaction of two or more loci. In the genus , has the dominant allele at the locus that causes hybrid lethality in F hybrid seedlings by interaction with allele(s). Here, we mapped the locus using the F population segregating for the allele derived from the interspecific cross between and . To map , several DNA markers including random amplified polymorphic DNA, amplified fragment length polymorphism, and simple sequence repeat markers, were used. Additionally, DNA markers were developed based on disease resistance gene homologs identified from the genome sequence of . Linkage analysis revealed that was located between two cleaved amplified polymorphic sequence markers Nb14-CAPS and NbRGH1-CAPS at a distance of 10.8 and 10.9 cM, respectively. The distance between these markers was equivalent to a 682 kb interval in the genome sequence of .

Citing Articles

Genetic Cause of Hybrid Lethality Observed in Reciprocal Interspecific Crosses between and .

Tezuka T, Nagai S, Matsuo C, Okamori T, Iizuka T, Marubashi W Int J Mol Sci. 2024; 25(2).

PMID: 38279225 PMC: 10817076. DOI: 10.3390/ijms25021226.

Genome-wide association study of leaf photosynthesis using a high-throughput gas exchange system in rice.

Honda S, Imamura A, Seki Y, Chigira K, Iwasa M, Hayami K Photosynth Res. 2023; 159(1):17-28.

PMID: 38112862 DOI: 10.1007/s11120-023-01065-3.

Understanding and overcoming hybrid lethality in seed and seedling stages as barriers to hybridization and gene flow.

He H, Shiragaki K, Tezuka T Front Plant Sci. 2023; 14:1219417.

PMID: 37476165 PMC: 10354522. DOI: 10.3389/fpls.2023.1219417.

The hybrid lethality of interspecific F hybrids of : a clue to understanding hybrid inviability-a major obstacle to wide hybridization and introgression breeding of plants.

Mino M, Tezuka T, Shomura S Mol Breed. 2023; 42(2):10.

PMID: 37309322 PMC: 10248639. DOI: 10.1007/s11032-022-01279-8.

Parental Genome Imbalance Causes Hybrid Seed Lethality as Well as Ovary Abscission in Interspecific and Interploidy Crosses in .

He H, Sadahisa K, Yokoi S, Tezuka T Front Plant Sci. 2022; 13:899206.

PMID: 35665169 PMC: 9161172. DOI: 10.3389/fpls.2022.899206.

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