A Chemosensor Based on Gold Nanoparticles and Dithiothreitol (DTT) for Acrylamide Electroanalysis

Overview

Journal Nanomaterials (Basel)

Date 2021 Oct 23

PMID 34685051

Citations 1

Authors

Shahenvaz Alam

Shine Augustine

Tarun Narayan

John H T Luong

Bansi Dhar Malhotra

Sunil K Khare

Affiliations

Soon will be listed here.

Abstract

Rapid and simple electroanalysis of acrylamide (ACR) was feasible by a gold electrode modified with gold nanoparticles (AuNPs) and dithiothreitol (DTT) with enhanced detection sensitivity and selectivity. The roughness of bare gold (Au) increased from 0.03 μm to 0.04 μm when it was decorated with AuNPs. The self-assembly between DTT and AuNPs resulted in a surface roughness of 0.09 μm. The DTT oxidation occurred at +0.92 V. The Au/AuNPs/DTT surface exhibited a surface roughness of 0.24 μm after its exposure to ACR with repeated analysis. SEM imaging illustrated the formation of a polymer layer on the Au/AuNPs/DTT surface. Surface plasmon resonance analysis confirmed the presence of AuNPs and DTT on the gold electrode and the binding of ACR to the electrode's active surface area. The peak area obtained by differential pulse voltammetry was inversely proportional to the ACR concentrations. The limit of detection (LOD) and the limit of quantitation (LOQ) were estimated to be 3.11 × 10 M and 1 × 10 M, respectively, with wide linearity ranging from 1 × 10 M to 1 × 10 M. The estimated levels of ACR in potato chips and coffee samples by the sensor were in agreement with those of high-performance liquid chromatography.

Citing Articles

An Effective Label-Free Electrochemical Aptasensor Based on Gold Nanoparticles for Gluten Detection.

Svigelj R, Zuliani I, Grazioli C, Dossi N, Toniolo R Nanomaterials (Basel). 2022; 12(6).

PMID: 35335800 PMC: 8953296. DOI: 10.3390/nano12060987.

References

Zyzak D, Sanders R, Stojanovic M, Tallmadge D, Eberhart B, Ewald D . Acrylamide formation mechanism in heated foods. J Agric Food Chem. 2004; 51(16):4782-7. DOI: 10.1021/jf034180i. View

Cleland W . DITHIOTHREITOL, A NEW PROTECTIVE REAGENT FOR SH GROUPS. Biochemistry. 1964; 3:480-2. DOI: 10.1021/bi00892a002. View

Liang P, Canoura J, Yu H, Alkhamis O, Xiao Y . Dithiothreitol-Regulated Coverage of Oligonucleotide-Modified Gold Nanoparticles To Achieve Optimized Biosensor Performance. ACS Appl Mater Interfaces. 2018; 10(4):4233-4242. PMC: 5794567. DOI: 10.1021/acsami.7b16914. View

Ishizuka M, Fujioka K, Shibamoto T . Analysis of acrylamide in a complex matrix of polyacrylamide solutions treated by heat and ultraviolet light. J Agric Food Chem. 2008; 56(15):6093-6. DOI: 10.1021/jf073052h. View

Batra B, Lata S, Pundir C . Construction of an improved amperometric acrylamide biosensor based on hemoglobin immobilized onto carboxylated multi-walled carbon nanotubes/iron oxide nanoparticles/chitosan composite film. Bioprocess Biosyst Eng. 2013; 36(11):1591-9. DOI: 10.1007/s00449-013-0931-5. View

Yadav N, Chhillar A, Pundir C . Preparation, characterization and application of haemoglobin nanoparticles for detection of acrylamide in processed foods. Int J Biol Macromol. 2017; 107(Pt A):1000-1013. DOI: 10.1016/j.ijbiomac.2017.09.070. View

Bucur M, Bucur B, Radu G . Simple, selective and fast detection of acrylamide based on glutathione -transferase. RSC Adv. 2022; 8(42):23931-23936. PMC: 9081860. DOI: 10.1039/c8ra02252f. View

Zhang W, Yuan Z, Huang L, Kang J, Jiang R, Zhong H . Titanium Dioxide Photocatalytic Polymerization of Acrylamide for Gel Electrophoresis (TIPPAGE) of Proteins and Structural Identification by Mass Spectrometry. Sci Rep. 2016; 6:20981. PMC: 4750088. DOI: 10.1038/srep20981. View

Lee Y, Wu B, Raymond J, Zeng Y, Fang X, Wooley K . A genetically encoded acrylamide functionality. ACS Chem Biol. 2013; 8(8):1664-70. PMC: 3746000. DOI: 10.1021/cb400267m. View

10.

Sumner S, Fennell T, Moore T, Chanas B, Gonzalez F, Ghanayem B . Role of cytochrome P450 2E1 in the metabolism of acrylamide and acrylonitrile in mice. Chem Res Toxicol. 1999; 12(11):1110-6. DOI: 10.1021/tx990040k. View

11.

Xue Y, Li X, Li H, Zhang W . Quantifying thiol-gold interactions towards the efficient strength control. Nat Commun. 2014; 5:4348. DOI: 10.1038/ncomms5348. View

12.

Zahler W, Cleland W . A specific and sensitive assay for disulfides. J Biol Chem. 1968; 243(4):716-9. View

13.

Bortolomeazzi R, Munari M, Anese M, Verardo G . Rapid mixed mode solid phase extraction method for the determination of acrylamide in roasted coffee by HPLC-MS/MS. Food Chem. 2012; 135(4):2687-93. DOI: 10.1016/j.foodchem.2012.07.057. View

14.

Basch H, Cohen R, Ratner M . Interface geometry and molecular junction conductance: geometric fluctuation and stochastic switching. Nano Lett. 2005; 5(9):1668-75. DOI: 10.1021/nl050702s. View

15.

Krajewska A, Radecki J, Radecka H . A Voltammetric Biosensor Based on Glassy Carbon Electrodes Modified with Single-Walled Carbon Nanotubes/Hemoglobin for Detection of Acrylamide in Water Extracts from Potato Crisps. Sensors (Basel). 2016; 8(9):5832-5844. PMC: 3705533. DOI: 10.3390/s8095832. View

16.

Kacar S, Vejselova D, Kutlu H, Sahinturk V . Acrylamide-derived cytotoxic, anti-proliferative, and apoptotic effects on A549 cells. Hum Exp Toxicol. 2017; 37(5):468-474. DOI: 10.1177/0960327117712386. View

17.

Kafouris D, Stavroulakis G, Christofidou M, Iakovou X, Christou E, Paikousis L . Determination of acrylamide in food using a UPLC-MS/MS method: results of the official control and dietary exposure assessment in Cyprus. Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2018; 35(10):1928-1939. DOI: 10.1080/19440049.2018.1508893. View

18.

Dybing E, Sanner T . Risk assessment of acrylamide in foods. Toxicol Sci. 2003; 75(1):7-15. DOI: 10.1093/toxsci/kfg165. View

19.

Horcas I, Fernandez R, Gomez-Rodriguez J, Colchero J, Gomez-Herrero J, Baro A . WSXM: a software for scanning probe microscopy and a tool for nanotechnology. Rev Sci Instrum. 2007; 78(1):013705. DOI: 10.1063/1.2432410. View

20.

Bandarra S, Fernandes A, Magro I, Guerreiro P, Pingarilho M, Churchwell M . Mechanistic insights into the cytotoxicity and genotoxicity induced by glycidamide in human mammary cells. Mutagenesis. 2013; 28(6):721-9. DOI: 10.1093/mutage/get052. View