Assessing the Biocompatibility of Multi-Anchored Glycoconjugate Functionalized Iron Oxide Nanoparticles in a Normal Human Colon Cell Line CCD-18Co

Overview

Journal Nanomaterials (Basel)

Date 2021 Oct 23

PMID 34684906

Citations 1

Authors

Yash S Raval

Anna Samstag

Cedric Taylor

Guohui Huang

Olin Thompson Mefford

Tzuen-Rong Jeremy Tzeng

Affiliations

Soon will be listed here.

Abstract

We have previously demonstrated that iron oxide nanoparticles with dopamine-anchored heterobifunctional polyethylene oxide (PEO) polymer, namely PEO-IONPs, and bio-functionalized with sialic-acid specific glycoconjugate moiety (Neu5Ac(α2-3)Gal(β1-4)-Glcβ-sp), namely GM3-IONPs, can be effectively used as antibacterial agents against target . In this study, we evaluated the biocompatibility of PEO-IONPs and GM3-IONPs in a normal human colon cell line CCD-18Co via measuring cell proliferation, membrane integrity, and intracellular adenosine triphosphate (ATP), glutathione GSH, dihydrorhodamine (DHR) 123, and caspase 3/7 levels. PEO-IONPs caused a significant decrease in cell viability at concentrations above 100 μg/mL whereas GM3-IONPs did not cause a significant decrease in cell viability even at the highest dose of 500 μg/mL. The ATP synthase activity of CCD-18Co was significantly diminished in the presence of PEO-IONPs but not GM3-IONPs. PEO-IONPs also compromised the membrane integrity of CCD-18Co. In contrast, cells exposed to GM3-IONPs showed significantly different cell morphology, but with no apparent membrane damage. The interaction of PEO-IONPs or GM3-IONPs with CCD-18Co resulted in a substantial decrease in the intracellular GSH levels in a time- and concentration-dependent manner. Conversely, levels of DHR-123 increased with IONP concentrations. Levels of caspase 3/7 proteins were found to be significantly elevated in cells exposed to PEO-IONPs. Based on the results, we assume GM3-IONPs to be biocompatible with CCD-18Co and could be further evaluated for selective killing of pathogens in vivo.

Citing Articles

Toxicity and magnetometry evaluation of the uptake of core-shell maghemite-silica nanoparticles by neuroblastoma cells.

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References

Creixell M, Bohorquez A, Torres-Lugo M, Rinaldi C . EGFR-targeted magnetic nanoparticle heaters kill cancer cells without a perceptible temperature rise. ACS Nano. 2011; 5(9):7124-9. DOI: 10.1021/nn201822b. View

Wahajuddin , Arora S . Superparamagnetic iron oxide nanoparticles: magnetic nanoplatforms as drug carriers. Int J Nanomedicine. 2012; 7:3445-71. PMC: 3405876. DOI: 10.2147/IJN.S30320. View

Reddy L, Arias J, Nicolas J, Couvreur P . Magnetic nanoparticles: design and characterization, toxicity and biocompatibility, pharmaceutical and biomedical applications. Chem Rev. 2012; 112(11):5818-78. DOI: 10.1021/cr300068p. View

Gabor F, Bogner E, Weissenboeck A, Wirth M . The lectin-cell interaction and its implications to intestinal lectin-mediated drug delivery. Adv Drug Deliv Rev. 2004; 56(4):459-80. DOI: 10.1016/j.addr.2003.10.015. View

Riedl S, Shi Y . Molecular mechanisms of caspase regulation during apoptosis. Nat Rev Mol Cell Biol. 2004; 5(11):897-907. DOI: 10.1038/nrm1496. View

Gao J, Gu H, Xu B . Multifunctional magnetic nanoparticles: design, synthesis, and biomedical applications. Acc Chem Res. 2009; 42(8):1097-107. DOI: 10.1021/ar9000026. View

Gupta A, Gupta M . Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications. Biomaterials. 2005; 26(18):3995-4021. DOI: 10.1016/j.biomaterials.2004.10.012. View

Park J, An K, Hwang Y, Park J, Noh H, Kim J . Ultra-large-scale syntheses of monodisperse nanocrystals. Nat Mater. 2004; 3(12):891-5. DOI: 10.1038/nmat1251. View

Naqvi S, Samim M, Abdin M, Jalees Ahmed F, Maitra A, Prashant C . Concentration-dependent toxicity of iron oxide nanoparticles mediated by increased oxidative stress. Int J Nanomedicine. 2010; 5:983-9. PMC: 3010160. DOI: 10.2147/IJN.S13244. View

10.

Wu X, Tan Y, Mao H, Zhang M . Toxic effects of iron oxide nanoparticles on human umbilical vein endothelial cells. Int J Nanomedicine. 2010; 5:385-99. PMC: 2950396. DOI: 10.2147/ijn.s10458. View

11.

Hauser A, Anderson K, Hilt J . Peptide conjugated magnetic nanoparticles for magnetically mediated energy delivery to lung cancer cells. Nanomedicine (Lond). 2016; 11(14):1769-85. PMC: 5827791. DOI: 10.2217/nnm-2016-0050. View

12.

Lu A, Salabas E, Schuth F . Magnetic nanoparticles: synthesis, protection, functionalization, and application. Angew Chem Int Ed Engl. 2007; 46(8):1222-44. DOI: 10.1002/anie.200602866. View

13.

Jain T, Richey J, Strand M, Leslie-Pelecky D, Flask C, Labhasetwar V . Magnetic nanoparticles with dual functional properties: drug delivery and magnetic resonance imaging. Biomaterials. 2008; 29(29):4012-21. PMC: 2593647. DOI: 10.1016/j.biomaterials.2008.07.004. View

14.

Huang J, Bu L, Xie J, Chen K, Cheng Z, Li X . Effects of nanoparticle size on cellular uptake and liver MRI with polyvinylpyrrolidone-coated iron oxide nanoparticles. ACS Nano. 2010; 4(12):7151-60. PMC: 3011031. DOI: 10.1021/nn101643u. View

15.

Escamilla-Rivera V, Uribe-Ramirez M, Gonzalez-Pozos S, Lozano O, Lucas S, De Vizcaya-Ruiz A . Protein corona acts as a protective shield against Fe3O4-PEG inflammation and ROS-induced toxicity in human macrophages. Toxicol Lett. 2015; 240(1):172-84. DOI: 10.1016/j.toxlet.2015.10.018. View

16.

Petri-Fink A, Chastellain M, Juillerat-Jeanneret L, Ferrari A, Hofmann H . Development of functionalized superparamagnetic iron oxide nanoparticles for interaction with human cancer cells. Biomaterials. 2004; 26(15):2685-94. DOI: 10.1016/j.biomaterials.2004.07.023. View

17.

Russell-Jones G, Veitch H, Arthur L . Lectin-mediated transport of nanoparticles across Caco-2 and OK cells. Int J Pharm. 1999; 190(2):165-74. DOI: 10.1016/s0378-5173(99)00254-9. View

18.

Wydra R, Rychahou P, Evers B, Anderson K, Dziubla T, Hilt J . The role of ROS generation from magnetic nanoparticles in an alternating magnetic field on cytotoxicity. Acta Biomater. 2015; 25:284-90. PMC: 4562873. DOI: 10.1016/j.actbio.2015.06.037. View

19.

Choi K, Nam K, Malkinski L, Choi E, Jung J, Park B . Size-Dependent Photodynamic Anticancer Activity of Biocompatible Multifunctional Magnetic Submicron Particles in Prostate Cancer Cells. Molecules. 2016; 21(9). PMC: 6273714. DOI: 10.3390/molecules21091187. View

20.

Nicholson D, Thornberry N . Caspases: killer proteases. Trends Biochem Sci. 1997; 22(8):299-306. DOI: 10.1016/s0968-0004(97)01085-2. View