Microarray Analysis of Paramylon, Isolated from Euglena Gracilis EOD-1, and Its Effects on Lipid Metabolism in the Ileum and Liver in Diet-Induced Obese Mice

Overview

Journal Nutrients

Date 2021 Oct 23

PMID 34684408

Citations 2

Authors

Seiichiro Aoe

Chiemi Yamanaka

Kento Mio

Affiliations

Soon will be listed here.

Abstract

We previously showed that supplementation of a high fat diet with paramylon (PM) reduces the postprandial glucose rise, serum total and LDL cholesterol levels, and abdominal fat accumulation in mice. The purpose of this study was to explore the underlying mechanism of PM using microarray analysis. Male mice (C57BL/BL strain) were fed an experimental diet (50% fat energy) containing 5% PM isolated from EOD-1 for 12 weeks. After confirming that PM had an improving effect on lipid metabolism, we assessed ileal and hepatic mRNA expression using DNA microarray and subsequent analysis by gene ontology (GO) classification and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. The results suggested that dietary supplementation with PM resulted in decreased abdominal fat accumulation and serum LDL cholesterol concentrations via suppression of the digestion and absorption pathway in the ileum and activation of the hepatic PPAR signaling pathway. Postprandial glucose rise was reduced in mice fed PM, whereas changes in the glucose metabolism pathway were not detected in GO classification and KEGG pathway analysis. PM intake might enhance serum secretory immunoglobulin A concentrations via promotion of the immunoglobulin production pathway in the ileum.

Citing Articles

Effects of paramylon-rich EOD-1 powder on visceral fat obesity in moderately obese Japanese adults: A randomized, double-blind, placebo-controlled, parallel-group trial.

Aoe S, Kawano T, Naito J, Nishida N, Takahashi M Food Sci Nutr. 2023; 11(2):953-962.

PMID: 36789056 PMC: 9922139. DOI: 10.1002/fsn3.3130.

Beta-Glucan in Foods and Health Benefits.

Aoe S Nutrients. 2022; 14(1).

PMID: 35010971 PMC: 8746796. DOI: 10.3390/nu14010096.

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