Decreased Expression of Cytotoxic Proteins in Decidual CD8 T Cells in Preeclampsia

Overview

Journal Biology (Basel)

Publisher MDPI

Specialty Biology

Date 2021 Oct 23

PMID 34681139

Citations 4

Authors

Violeta Soljic

Maja Barbaric

Martina Vukoja

Marina Curlin

Martina Orlovic Vlaho

Edita Cerni Obrdalj

Lidija Lasic Arapovic

Daniela Bevanda Glibo

Katarina Vukojevic

Affiliations

Soon will be listed here.

Abstract

In our study, we aimed to establish expression of cytotoxic CD8 T cells in the decidua basalis and the maternal peripheral blood (mPBL) of severe and mild preeclampsia (PE) and compare to healthy pregnancies. Decidual tissue and mPBL of 10 women with mild PE, 10 women with severe PE, and 20 age-matched healthy pregnancy controls were analyzed by double immunofluorescence and qPCR, respectively. By double immunofluorescence staining, we found a decreased total number of cells/mm in decidua basalis of granulysin (GNLY) ( ˂ 0.0001), granzyme B (GzB)( ˂ 0.0001), GzBCD8( ˂ 0.0001), perforin (PRF1) ( ˂ 0.0001), and PRF1CD8 ( ˂ 0.01) in the severe PE compared to control group. Additionally, we noticed the trend of lower mRNA expression for GNLY, granzyme A (GZMA), GzB, and PRF1 in CD8 T cells of mPBL in mild and severe PE, with the latter marker statistically decreased in severe PE ( ˂ 0.001). Forkhead box P3 (FOXP3) mRNA in CD8 T cells mPBL was increased in mild PE ( ˂ 0.001) compared to controls. In conclusion, severe PE is characterized by altered expression of cytotoxic CD8 T cells in decidua and mPBL, suggesting their role in pathophysiology of PE and fetal-maternal immune tolerance.

Citing Articles

Expression of IMP3 and LIN28A RNA-Binding Proteins in Placentas of Patients with Pre-Eclampsia with and without Severe Features.

Barbaric M, Vukojevic K, Kolobaric A, Orlovic Vlaho M, Kresic T, Soljic V Biomedicines. 2024; 12(4).

PMID: 38672233 PMC: 11048248. DOI: 10.3390/biomedicines12040879.

Effect of Granzyme K, FasL and Interferon-γ Expression in Placentas with Preeclampsia.

Vukoja M, Curlin M, Vukojevic K, Jelic-Knezovic N, Kolobaric A, Orlovic Vlaho M Biomedicines. 2024; 12(4).

PMID: 38672196 PMC: 11048069. DOI: 10.3390/biomedicines12040842.

Bioinformatic analysis reveals lysosome-related biomarkers and molecular subtypes in preeclampsia: novel insights into the pathogenesis of preeclampsia.

Chen Y, Liu M, Wang Y Front Genet. 2023; 14:1228110.

PMID: 37576559 PMC: 10416227. DOI: 10.3389/fgene.2023.1228110.

Prognostic and predictive significance of VEGF, CD31, and Ang-1 in patients with metastatic clear cell renal cell carcinoma treated with first-line sunitinib.

Kraljevic M, Marijanovic I, Barbaric M, Sokolovic E, Bukva M, Ceric T Biomol Biomed. 2022; 23(1):161-169.

PMID: 35674770 PMC: 9901909. DOI: 10.17305/bjbms.2022.7675.

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