Histological Evaluation of Gingival and Intrabony Periodontal Defects Treated with Platelet-rich Fibrin Using Different Protocols: A Canine Study

Overview

Journal Oral Health Prev Dent

Date 2021 Oct 21

PMID 34673845

Citations 2

Authors

Yoshinori Shirakata

Kotaro Sena

Toshiaki Nakamura

Yukiya Shinohara

Takatomo Imafuji

Fumiaki Setoguchi

Kazuyuki Noguchi

Tomoyuki Kawase

Richard J Miron

Affiliations

Soon will be listed here.

Abstract

Purpose: To histologically compare the effects of platelet-rich fibrin (PRF) produced using different protocols on periodontal wound healing/regeneration in periodontal defects in dogs.

Materials And Methods: Dehiscence-type gingival recession and two-wall intrabony defects were created bilaterally in the maxillary canines and mandibular premolars, respectively, in four beagle dogs. The recession defects were randomly treated with coronally advanced flap (CAF) alone, CAF and PRF produced via fixed-angle centrifugation (F-PRF; Leukocyte and PRF (L-PRF) protocol) or CAF and PRF produced via horizontal centrifugation (H-PRF). After 2 weeks, the two-wall intrabony defects were randomly treated as follows: open flap debridement (OFD), OFD + F-PRF, OFD + H-PRF and OFD + heated albumin with PRF using bio-heat technologies (Alb-PRF). Eight weeks after the 2nd reconstructive surgery, the animals were euthanised for histological evaluation.

Results: In the PRF-applied defects, new bone and new cementum formation occurred to varying degrees regardless of the protocols used to produce PRF. Particularly in the two-wall intrabony defects, new bone formation extended from the host bone toward the coronal region of the defects in the H-PRF applied sites compared with those in the OFD, F-PRF and Alb-PRF groups, and the H-PRF group showed the greatest amount of newly formed cementum.

Conclusion: PRF induced periodontal regeneration in gingival recession and two-wall intrabony defects in dogs. Further studies are needed to determine the optimal protocol for obtaining predictable periodontal regeneration in periodontal defects in humans.

Citing Articles

Investigating the Biological Efficacy of Albumin-Enriched Platelet-Rich Fibrin (Alb-PRF): A Study on Cytokine Dynamics and Osteoblast Behavior.

Stellet Lourenco E, Rodrigues Santiago Rocha N, de Lima Barbosa R, Mello-Machado R, De Souza Lima V, Leite P Int J Mol Sci. 2024; 25(21).

PMID: 39519084 PMC: 11547010. DOI: 10.3390/ijms252111531.

The Association of Nanostructured Carbonated Hydroxyapatite with Denatured Albumin and Platelet-Rich Fibrin: Impacts on Growth Factors Release and Osteoblast Behavior.

de Lima Barbosa R, Rodrigues Santiago Rocha N, Stellet Lourenco E, De Souza Lima V, Mavropoulos E, Mello-Machado R J Funct Biomater. 2024; 15(1).

PMID: 38248685 PMC: 10817063. DOI: 10.3390/jfb15010018.

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