Endophytic Bacterial Communities of Leaves During Leaf Developmental Period

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2021 Oct 21

PMID 34671323

Citations 12

Authors

Yan Deng

Haonan Huang

Fangying Lei

Shaodong Fu

Kai Zou

Shuangfei Zhang

Xueduan Liu

Luhua Jiang

Hongwei Liu

Bo Miao

Yili Liang

Affiliations

Soon will be listed here.

Abstract

Plant-specialized secondary metabolites have ecological functions in mediating interactions between plants and their entophytes. In this study, high-throughput gene sequencing was used to analyze the composition and abundance of bacteria from leaves at five different sampling times. The results indicated that the bacterial community structure varied during leaf developmental stage. Bacterial diversity was observed to be the highest at T2 stage and the lowest at T1 stage. Proteobacteria, Firmicutes, Actinobacteria, Chloroflexi, Cyanobacteria, and Bacteroidetes were found as the dominant phyla. The major genera also showed consistency across sampling times, but there was a significant variation in their abundance, such as , , and Significant correlations were observed between endophytic bacteria and flavonoids. Especially, showed a significant positive correlation with quercetin, and changes in the abundance of also showed a strong correlation with flavonoid content. In order to determine the effect of flavonoids on endophytic bacteria of leaves, an extracorporeal culture of related strains (a strain of and a strain of ) was performed, and it was found that the effect of flavonoids on them remained consistent. The predicted result of Tax4Fun2 revealed that flavonoids might lead to a lower abundance of endophytic microorganisms, which further proved the correlation between bacterial communities and flavonoids. This study provided the first insight into the bacterial community composition during the development of leaves and the correlation between the endophytic bacteria and flavonoids.

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References

Li W, Godzik A . Cd-hit: a fast program for clustering and comparing large sets of protein or nucleotide sequences. Bioinformatics. 2006; 22(13):1658-9. DOI: 10.1093/bioinformatics/btl158. View

Hasler A, Gross G, Meier B, Sticher O . Complex flavonol glycosides from the leaves of Ginkgo biloba. Phytochemistry. 1992; 31(4):1391-4. DOI: 10.1016/0031-9422(92)80298-s. View

Cai H, Li H, Yan X, Sun B, Zhang Q, Yan M . Metabolomic analysis of biochemical changes in the plasma and urine of first-episode neuroleptic-naïve schizophrenia patients after treatment with risperidone. J Proteome Res. 2012; 11(8):4338-50. DOI: 10.1021/pr300459d. View

Moran N . Symbiosis as an adaptive process and source of phenotypic complexity. Proc Natl Acad Sci U S A. 2007; 104 Suppl 1:8627-33. PMC: 1876439. DOI: 10.1073/pnas.0611659104. View

Deng Y, He Z, Xu M, Qin Y, Van Nostrand J, Wu L . Elevated carbon dioxide alters the structure of soil microbial communities. Appl Environ Microbiol. 2012; 78(8):2991-5. PMC: 3318805. DOI: 10.1128/AEM.06924-11. View

Li P, Sun J, Xie X, Li Z, Huang B, Zhang G . Stress response and tolerance to perfluorooctane sulfonate (PFOS) in lettuce (Lactuca sativa). J Hazard Mater. 2020; 404(Pt B):124213. DOI: 10.1016/j.jhazmat.2020.124213. View

Huang Y . Effect of Host, Environment and Fungal Growth on Fungal Leaf Endophyte Communities in Taiwan. J Fungi (Basel). 2020; 6(4). PMC: 7712724. DOI: 10.3390/jof6040244. View

Cushnie T, Lamb A . Antimicrobial activity of flavonoids. Int J Antimicrob Agents. 2005; 26(5):343-56. PMC: 7127073. DOI: 10.1016/j.ijantimicag.2005.09.002. View

Tetali S, Acharya S, Ankari A, Nanakram V, Raghavendra A . Metabolomics of Withania somnifera (L.) Dunal: Advances and applications. J Ethnopharmacol. 2020; 267:113469. DOI: 10.1016/j.jep.2020.113469. View

10.

Lebeis S, Herrera Paredes S, Lundberg D, Breakfield N, Gehring J, McDonald M . PLANT MICROBIOME. Salicylic acid modulates colonization of the root microbiome by specific bacterial taxa. Science. 2015; 349(6250):860-4. DOI: 10.1126/science.aaa8764. View

11.

Asshauer K, Wemheuer B, Daniel R, Meinicke P . Tax4Fun: predicting functional profiles from metagenomic 16S rRNA data. Bioinformatics. 2015; 31(17):2882-4. PMC: 4547618. DOI: 10.1093/bioinformatics/btv287. View

12.

Papke R, Gogarten J . Ecology. How bacterial lineages emerge. Science. 2012; 336(6077):45-6. DOI: 10.1126/science.1219241. View

13.

Meyer K, Leveau J . Microbiology of the phyllosphere: a playground for testing ecological concepts. Oecologia. 2011; 168(3):621-9. PMC: 3277708. DOI: 10.1007/s00442-011-2138-2. View

14.

Fort T, Pauvert C, Zanne A, Ovaskainen O, Caignard T, Barret M . Maternal effects shape the seed mycobiome in Quercus petraea. New Phytol. 2020; 230(4):1594-1608. DOI: 10.1111/nph.17153. View

15.

Passera A, Rossato M, Oliver J, Battelli G, Shahzad G, Cosentino E . Characterization of Lysinibacillus fusiformis strain S4C11: In vitro, in planta, and in silico analyses reveal a plant-beneficial microbe. Microbiol Res. 2020; 244:126665. DOI: 10.1016/j.micres.2020.126665. View

16.

Wang L, Xiong F, Yang L, Xiao Y, Zhou G . A Seasonal Change of Active Ingredients and Mineral Elements in Root of Astragalus membranaceus in the Qinghai-Tibet Plateau. Biol Trace Elem Res. 2020; 199(10):3950-3959. DOI: 10.1007/s12011-020-02486-0. View

17.

Liu P, Cai Y, Zhang J, Wang R, Li B, Weng Q . Antifungal activity of liquiritin in Phytophthora capsici comprises not only membrane-damage-mediated autophagy, apoptosis, and Ca reduction but also an induced defense responses in pepper. Ecotoxicol Environ Saf. 2020; 209:111813. DOI: 10.1016/j.ecoenv.2020.111813. View

18.

Reitznerova A, Sulekova M, Nagy J, Marcincak S, Semjon B, certik M . Lipid Peroxidation Process in Meat and Meat Products: A Comparison Study of Malondialdehyde Determination between Modified 2-Thiobarbituric Acid Spectrophotometric Method and Reverse-Phase High-Performance Liquid Chromatography. Molecules. 2017; 22(11). PMC: 6150165. DOI: 10.3390/molecules22111988. View

19.

Banning N, Gleeson D, Grigg A, Grant C, Andersen G, Brodie E . Soil microbial community successional patterns during forest ecosystem restoration. Appl Environ Microbiol. 2011; 77(17):6158-64. PMC: 3165429. DOI: 10.1128/AEM.00764-11. View

20.

Brader G, Compant S, Vescio K, Mitter B, Trognitz F, Ma L . Ecology and Genomic Insights into Plant-Pathogenic and Plant-Nonpathogenic Endophytes. Annu Rev Phytopathol. 2017; 55:61-83. DOI: 10.1146/annurev-phyto-080516-035641. View