Detection of the Major Pertussis Toxin Substrate of Human Leukocytes with Antisera Raised Against Synthetic Peptides

Overview

Journal FEBS Lett

Specialty Biochemistry

Date 1986 Dec 15

PMID 3466805

Citations 15

Authors

J Falloon

H Malech

G Milligan

C Unson

R Kahn

P Goldsmith

A Spiegel

Affiliations

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Abstract

Antisera raised against the carboxy-terminal decapeptide (KENLKDCGLF) of transducin-alpha detected the 40 kDa, major pertussis toxin substrate of human neutrophils. The antisera also detected this protein in undifferentiated HL-60 and U937 cells, and revealed an approx. 2-fold increase in protein/mg membrane protein with differentiation into mature phagocytic cells. The results provide direct immunochemical evidence for the presence of a novel, pertussis toxin-sensitive guanine nucleotide-binding protein in human leukocytes.

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