Molecular Characterization of a Tolerant Saline-Alkali Phosphatidate Phosphatase That Confers NaCl and Sorbitol Tolerance

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2021 Oct 15

PMID 34650539

Authors

Jingang Wang

Qinghua Shan

Ye Ran

Dexiang Sun

Haizhen Zhang

Jinzhu Zhang

Shufang Gong

Aimin Zhou

Kun Qiao

Affiliations

Soon will be listed here.

Abstract

The gene encoding a putative () from tolerant saline-alkali (TSA) , , was identified from a yeast cDNA library constructed from TSA after a NaCl treatment. expressed in yeast enhanced its tolerance to NaCl and sorbitol. The ChPAP protein from a GFP-tagged construct localized to the plasma membrane and the lumen of vacuoles. The relative transcript levels of in cells were strongly induced by NaCl and sorbitol as assessed by northern blot analyses. Thus, ChPAP may play important roles in promoting Na-ion movement into the cell and maintaining the cytoplasmic ion balance. In addition, ChPAP may catalyze diacylglycerol pyrophosphate to phosphatidate in vacuoles.

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