Comparison of Apical Extrusion of Bacteria After Glide Path Preparation Between Manual K File, One G Rotary, and WaveOne Gold Glider Reciprocation Preparations

Overview

Journal Eur Endod J

Publisher Kare Publishing

Specialty Dentistry

Date 2021 Oct 15

PMID 34650017

Citations 1

Authors

Nicole Low

Saw Zhen Jie

Shekhar Bhatia

Fabian Davamani

Venkateshbabu Nagendrababu

Affiliations

Soon will be listed here.

Abstract

Objective: To compare the amount of apically extruded bacteria between hand-filed preparations, rotary and reciprocation glide path preparations in curved canals of extracted teeth infected with Enterococcus faecalis.

Methods: Forty mandibular first molar teeth were decoronated, fitted into rubber stoppers and fixed onto glass vials. The mesiobuccal canals from mandibular first molar teeth were infected with Enterococcus faecalis, then randomly assigned to one of five groups for glide path preparation: manual stainless-steel file (K-files), rotary file (One G), reciprocating file (WaveOne Gold Glider) and two control groups. After glide path preparation, 0.01 mL of saline was taken from the experimental vials. The solution was plated on tryptic soy agar and colonies of bacteria were counted as colony-forming units. The results were analysed statistically using Kruskal-Wallis and post hoc Mann-Whitney U tests.

Results: The manual K-file group was associated with significantly more bacteria extrusion compared to the rotary and reciprocating groups (P<0.05). However, no significant difference occurred between rotary and reciprocation instruments.

Conclusion: All instrumentation techniques resulted in a measurable amount of apical extrusion of bacteria. Manual K-files extruded the highest quantity of bacteria compared to One G rotary file and WaveOne Gold Glider reciprocation file during glide path preparation.

Citing Articles

Comparison of Debris Extrusion and Preparation Time by Traverse, R-Motion Glider C, and Other Glide Path Systems in Severely Curved Canals.

Al Omari T, Hassouneh L, Albashaireh K, Dkmak A, Albanna R, Al-Mohammed A Int J Dent. 2025; 2025:6619901.

PMID: 40041725 PMC: 11879535. DOI: 10.1155/ijod/6619901.

Glide Path in Endodontics: A Literature Review of Current Knowledge.

Lup V, Malvicini G, Gaeta C, Grandini S, Ciavoi G Dent J (Basel). 2024; 12(8).

PMID: 39195101 PMC: 11352993. DOI: 10.3390/dj12080257.

References

Alves F, Paiva P, Marceliano-Alves M, Cabreira L, Lima K, Siqueira Jr J . Bacteria and Hard Tissue Debris Extrusion and Intracanal Bacterial Reduction Promoted by XP-endo Shaper and Reciproc Instruments. J Endod. 2018; 44(7):1173-1178. DOI: 10.1016/j.joen.2018.04.007. View

Beeson T, Hartwell G, Thornton J, Gunsolley J . Comparison of debris extruded apically in straight canals: conventional filing versus profile .04 Taper series 29. J Endod. 1998; 24(1):18-22. DOI: 10.1016/S0099-2399(98)80206-9. View

Tanalp J, Gungor T . Apical extrusion of debris: a literature review of an inherent occurrence during root canal treatment. Int Endod J. 2013; 47(3):211-21. DOI: 10.1111/iej.12137. View

Ahn S, Kim H, Kim E . Kinematic Effects of Nickel-Titanium Instruments with Reciprocating or Continuous Rotation Motion: A Systematic Review of In Vitro Studies. J Endod. 2016; 42(7):1009-17. DOI: 10.1016/j.joen.2016.04.002. View

Shen Y, Stojicic S, Haapasalo M . Antimicrobial efficacy of chlorhexidine against bacteria in biofilms at different stages of development. J Endod. 2011; 37(5):657-61. DOI: 10.1016/j.joen.2011.02.007. View

Myers G, Montgomery S . A comparison of weights of debris extruded apically by conventional filing and Canal Master techniques. J Endod. 1991; 17(6):275-9. DOI: 10.1016/S0099-2399(06)81866-2. View

Adiguzel M, Yilmaz K, Tufenkci P . Comparison of postoperative pain intensity after using reciprocating and continuous rotary glide path systems: a randomized clinical trial. Restor Dent Endod. 2019; 44(1):e9. PMC: 6387895. DOI: 10.5395/rde.2019.44.e9. View

Ozyurek T, Uslu G, Gundogar M, Yilmaz K, Grande N, Plotino G . Comparison of cyclic fatigue resistance and bending properties of two reciprocating nickel-titanium glide path files. Int Endod J. 2018; 51(9):1047-1052. DOI: 10.1111/iej.12911. View

West J . The endodontic Glidepath: "Secret to rotary safety". Dent Today. 2010; 29(9):86, 88, 90-3. View

10.

Aksel H, Kucukkaya Eren S, Cakar A, Serper A, Ozkuyumcu C, Azim A . Effect of Instrumentation Techniques and Preparation Taper on Apical Extrusion of Bacteria. J Endod. 2017; 43(6):1008-1010. DOI: 10.1016/j.joen.2017.01.014. View

11.

Dagna A, Abed R, Hussain S, Abu-Tahun I, Visai L, Bertoglio F . Comparison of apical extrusion of intracanal bacteria by various glide-path establishing systems: an study. Restor Dent Endod. 2017; 42(4):316-323. PMC: 5682148. DOI: 10.5395/rde.2017.42.4.316. View

12.

Siqueira Jr J, Rocas I . Polymerase chain reaction-based analysis of microorganisms associated with failed endodontic treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2004; 97(1):85-94. DOI: 10.1016/s1079-2104(03)00353-6. View

13.

Teixeira J, Cunha F, Jesus R, Silva E, Fidel S, Sassone L . Influence of working length and apical preparation size on apical bacterial extrusion during reciprocating instrumentation. Int Endod J. 2014; 48(7):648-53. DOI: 10.1111/iej.12357. View

14.

Paleker F, van der Vyver P . Glide Path Enlargement of Mandibular Molar Canals by Using K-files, the ProGlider File, and G-Files: A Comparative Study of the Preparation Times. J Endod. 2017; 43(4):609-612. DOI: 10.1016/j.joen.2016.11.025. View

15.

Gunes B, Yeter K . Effects of Different Glide Path Files on Apical Debris Extrusion in Curved Root Canals. J Endod. 2018; 44(7):1191-1194. DOI: 10.1016/j.joen.2018.04.012. View

16.

Turker S, Uzunoglu E, Aslan M . Evaluation of apically extruded bacteria associated with different nickel-titanium systems. J Endod. 2015; 41(6):953-5. DOI: 10.1016/j.joen.2015.02.009. View

17.

Ferraz C, Gomes N, Gomes B, Zaia A, Teixeira F, Souza-Filho F . Apical extrusion of debris and irrigants using two hand and three engine-driven instrumentation techniques. Int Endod J. 2001; 34(5):354-8. DOI: 10.1046/j.1365-2591.2001.00394.x. View

18.

DAmario M, Baldi M, Petricca R, De Angelis F, Abed R, DArcangelo C . Evaluation of a new nickel-titanium system to create the glide path in root canal preparation of curved canals. J Endod. 2013; 39(12):1581-4. DOI: 10.1016/j.joen.2013.06.037. View

19.

Topcuoglu H, Duzgun S, Akpek F, Topcuoglu G, Akti A . Influence of a glide path on apical extrusion of debris during canal preparation using single-file systems in curved canals. Int Endod J. 2015; 49(6):599-603. DOI: 10.1111/iej.12484. View

20.

Schneider S . A comparison of canal preparations in straight and curved root canals. Oral Surg Oral Med Oral Pathol. 1971; 32(2):271-5. DOI: 10.1016/0030-4220(71)90230-1. View