Effects of Yu Linzhu on Ovarian Function and Oocyte Mitochondria in Natural Aging Mice

Overview

Journal Aging (Albany NY)

Specialty Geriatrics

Date 2021 Oct 14

PMID 34648463

Citations 5

Authors

Zhen Yang

Mao-Lin Wei

Xiao-Ying Dong

Affiliations

Soon will be listed here.

Abstract

Objective: To study the effect of Yu Linzhu on ovarian function and mitochondria in natural aging mice.

Methods: Female BALB/c mice were selected as normal group at 7-8 weeks and natural aging group at 9 months. The natural aging group was divided into Yu Linzhu intervention group and non-intervention group by intragastric administration once a day for 6 weeks. The morphology and blood flow of ovary were observed by ultrasound. Ovarian morphology and follicle were observed by HE staining. Hormone levels were analyzed by ELISA. Serum oxidative stress were detected by radioimmunoassay. The distribution of mitochondria in oocytes was observed by fluorescence staining. The ultrastructure of oocytes and the morphology of mitochondria were observed under electron microscope. The mitochondrial membrane potential was detected by JC-1.

Results: Two groups of aging mice had serious disturbance of estrus cycle. The ovarian area of the mice in the aging non-intervention group was smaller than that in the normal group, and the ovarian area of the mice in the aging intervention group recovered. The ovarian blood flow was weak or even disappear in the aging non-intervention group, and the blood flow in the intervention group was improved. The ovarian volume of mice in the non-intervention group was smaller than that in the normal group. Some ovarian tissues were adhered to the surrounding tissues. While in the intervention group, the ovarian volume increased, the degree of adhesion decreased, the infiltration of ovarian interstitial lymphocytes decreased, and the zona pellucida recovered. Granular cell arrangement returned neatly, egg cell shape recover regular and the number also increased. In the non-intervention group, E2 (Estrogen), AMH (Anti-Mullerian hormone) decreased ( = 0.0092 and = 0.0334, respectively), FSH (Follicle stimulating hormone) increased ( < 0.0001). In the intervention group, FSH decreased ( = 0.0002), LH (luteinizing hormone) decreased and E2, AMH increased. In the non-intervention group, GSH-Px (Glutathione peroxidase) decreased ( = 0.0129), SOD (Superoxide dismutase) decreased, ROS (reactive oxidative species), MDA (Malondialdehyde) increased. In the aging intervention group, ROS, MDA decreased and GSH-Px increased. In the non-intervention group, mitochondrial expression was scattered at the concentrated distribution point, the length of mitochondria was mostly long and the average volume increased, the density decreased, the number decreased and some mitochondria fused, and lesions such as swelling, vacuolar degeneration and inclusion body formation, membrane potential decreased ( = 0.0002). In the aging intervention group, mitochondria were evenly distributed, the mitochondria were basically round, the distribution density was moderate, the inner ridge was clear, and the membrane potential of the aging intervention group increased.

Conclusion: Yu Linzhu can improve the ovarian function of natural aging mice by improving the mitochondrial function of oocytes.

Citing Articles

Mitochondria-Targeted Antioxidant MitoQ Improves In Vitro Maturation and Subsequent Embryonic Development from Culled Cows.

Feng Z, Shi J, Ren J, Luo L, Liu D, Guo Y Animals (Basel). 2024; 14(20).

PMID: 39457858 PMC: 11503749. DOI: 10.3390/ani14202929.

Yu Linzhu alleviates primary ovarian insufficiency in a rat model by improving proliferation and energy metabolism of granulosa cells through hif1α/cx43 pathway.

Ruan X, Wang P, Wei M, Yang Q, Dong X J Ovarian Res. 2024; 17(1):89.

PMID: 38671471 PMC: 11046760. DOI: 10.1186/s13048-024-01408-1.

A Systematic Review of In Vivo Studies of the Efficacy of Herbal Medicines for Anti-Aging in the Last Five Years.

Cho S, Lee H, Kwon S, Park S, Jung W, Moon S Pharmaceuticals (Basel). 2023; 16(3).

PMID: 36986547 PMC: 10054545. DOI: 10.3390/ph16030448.

Mitoquinone shifts energy metabolism to reduce ROS-induced oxeiptosis in female granulosa cells and mouse oocytes.

Tsui K, Li C Aging (Albany NY). 2023; 15(1):246-260.

PMID: 36626243 PMC: 9876626. DOI: 10.18632/aging.204475.

The role of oxidative stress in ovarian aging: a review.

Yan F, Zhao Q, Li Y, Zheng Z, Kong X, Shu C J Ovarian Res. 2022; 15(1):100.

PMID: 36050696 PMC: 9434839. DOI: 10.1186/s13048-022-01032-x.

References

Wang T, Zhang M, Jiang Z, Seli E . Mitochondrial dysfunction and ovarian aging. Am J Reprod Immunol. 2017; 77(5). DOI: 10.1111/aji.12651. View

Dumollard R, Carroll J, Duchen M, Campbell K, Swann K . Mitochondrial function and redox state in mammalian embryos. Semin Cell Dev Biol. 2009; 20(3):346-53. DOI: 10.1016/j.semcdb.2008.12.013. View

Yang L, Chen Y, Liu Y, Xing Y, Miao C, Zhao Y . The Role of Oxidative Stress and Natural Antioxidants in Ovarian Aging. Front Pharmacol. 2021; 11:617843. PMC: 7869110. DOI: 10.3389/fphar.2020.617843. View

May-Panloup P, Boucret L, Chao de la Barca J, Desquiret-Dumas V, Ferre-LHotellier V, Moriniere C . Ovarian ageing: the role of mitochondria in oocytes and follicles. Hum Reprod Update. 2016; 22(6):725-743. DOI: 10.1093/humupd/dmw028. View

Tilly J, Sinclair D . Germline energetics, aging, and female infertility. Cell Metab. 2013; 17(6):838-850. PMC: 3756096. DOI: 10.1016/j.cmet.2013.05.007. View

Emidio G, Falone S, Vitti M, DAlessandro A, Vento M, Di Pietro C . SIRT1 signalling protects mouse oocytes against oxidative stress and is deregulated during aging. Hum Reprod. 2014; 29(9):2006-17. DOI: 10.1093/humrep/deu160. View

Tatone C, Amicarelli F . The aging ovary--the poor granulosa cells. Fertil Steril. 2013; 99(1):12-17. DOI: 10.1016/j.fertnstert.2012.11.029. View

Miliotis S, Nicolalde B, Ortega M, Yepez J, Caicedo A . Forms of extracellular mitochondria and their impact in health. Mitochondrion. 2019; 48:16-30. DOI: 10.1016/j.mito.2019.02.002. View

Shirzeyli M, Amidi F, Shamsara M, Nazarian H, Eini F, Shirzeyli F . Exposing Mouse Oocytes to MitoQ During Maturation Improves Maturation and Developmental Competence. Iran J Biotechnol. 2021; 18(3):e2454. PMC: 8035425. DOI: 10.30498/IJB.2020.154641.2454. View

10.

Gilchrist R, Lane M, Thompson J . Oocyte-secreted factors: regulators of cumulus cell function and oocyte quality. Hum Reprod Update. 2008; 14(2):159-77. DOI: 10.1093/humupd/dmm040. View

11.

Sutton-McDowall M, Gilchrist R, Thompson J . The pivotal role of glucose metabolism in determining oocyte developmental competence. Reproduction. 2010; 139(4):685-95. DOI: 10.1530/REP-09-0345. View

12.

Barros M, da Cunha F, Oliveira G, Tahara E, Kowaltowski A . Yeast as a model to study mitochondrial mechanisms in ageing. Mech Ageing Dev. 2010; 131(7-8):494-502. DOI: 10.1016/j.mad.2010.04.008. View

13.

Ford J . Reduced quality and accelerated follicle loss with female reproductive aging - does decline in theca dehydroepiandrosterone (DHEA) underlie the problem?. J Biomed Sci. 2013; 20:93. PMC: 3878748. DOI: 10.1186/1423-0127-20-93. View

14.

Simsek-Duran F, Li F, Ford W, Swanson R, Jones Jr H, Castora F . Age-associated metabolic and morphologic changes in mitochondria of individual mouse and hamster oocytes. PLoS One. 2013; 8(5):e64955. PMC: 3669215. DOI: 10.1371/journal.pone.0064955. View