Monoclonal Antibodies from Humans with Mycobacterium Tuberculosis Exposure or Latent Infection Recognize Distinct Arabinomannan Epitopes

Overview

Journal Commun Biol

Specialty Biology

Date 2021 Oct 13

PMID 34642445

Citations 8

Authors

Elise Ishida

Devin T Corrigan

Ryan J Malonis

Daniel Hofmann

Tingting Chen

Anita G Amin

Delphi Chatterjee

Maju Joe

Todd L Lowary

Jonathan R Lai

Jacqueline M Achkar

Affiliations

Soon will be listed here.

Abstract

The surface polysacharide arabinomannan (AM) and related glycolipid lipoarabinomannan (LAM) play critical roles in tuberculosis pathogenesis. Human antibody responses to AM/LAM are heterogenous and knowledge of reactivity to specific glycan epitopes at the monoclonal level is limited, especially in individuals who can control M. tuberculosis infection. We generated human IgG mAbs to AM/LAM from B cells of two asymptomatic individuals exposed to or latently infected with M. tuberculosis. Here, we show that two of these mAbs have high affinity to AM/LAM, are non-competing, and recognize different glycan epitopes distinct from other anti-AM/LAM mAbs reported. Both mAbs recognize virulent M. tuberculosis and nontuberculous mycobacteria with marked differences, can be used for the detection of urinary LAM, and can detect M. tuberculosis and LAM in infected lungs. These mAbs enhance our understanding of the spectrum of antibodies to AM/LAM epitopes in humans and are valuable for tuberculosis diagnostic and research applications.

Citing Articles

The mycobacterial glycoside hydrolase LamH enables capsular arabinomannan release and stimulates growth.

Franklin A, Salgueiro V, Layton A, Sullivan R, Mize T, Vazquez-Iniesta L Nat Commun. 2024; 15(1):5740.

PMID: 38982040 PMC: 11233589. DOI: 10.1038/s41467-024-50051-3.

Serum antibody screening using glycan arrays.

Marglous S, Brown C, Padler-Karavani V, Cummings R, Gildersleeve J Chem Soc Rev. 2024; 53(5):2603-2642.

PMID: 38305761 PMC: 7616341. DOI: 10.1039/d3cs00693j.

The mycobacterial glycoside hydrolase LamH enables capsular arabinomannan release and stimulates growth.

Franklin A, Layton A, Mize T, Salgueiro V, Sullivan R, Benedict S bioRxiv. 2023; .

PMID: 37961452 PMC: 10634837. DOI: 10.1101/2023.10.26.563968.

Features and protective efficacy of human mAbs targeting Mycobacterium tuberculosis arabinomannan.

Liu Y, Chen T, Zhu Y, Furey A, Lowary T, Chan J JCI Insight. 2023; 8(20.

PMID: 37733444 PMC: 10619501. DOI: 10.1172/jci.insight.167960.

Impact of Mycobacterium tuberculosis Glycolipids on the CD4+ T Cell-Macrophage Immunological Synapse.

Mwebaza I, Shaw R, Li Q, Fletcher S, Achkar J, Harding C J Immunol. 2023; 211(9):1385-1396.

PMID: 37695687 PMC: 10579150. DOI: 10.4049/jimmunol.2300107.

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