Analyzing the Whole-transcriptome Profiles of NcRNAs and Predicting the Competing Endogenous RNA Networks in Cervical Cancer Cell Lines with Cisplatin Resistance

Overview

Journal Cancer Cell Int

Publisher Biomed Central

Date 2021 Oct 13

PMID 34641878

Citations 2

Authors

Huimin Lv

Shanshan Jin

Binbin Zou

Yuxiang Liang

Jun Xie

Suhui Wu

Affiliations

Soon will be listed here.

Abstract

Background: Cervical cancer (CC) is one of the most common malignant tumors in women. In order to identify the functional roles and the interaction between mRNA and non-coding RNA (ncRNA, including lncRNA, circRNA and miRNA) in CC cisplatin (DDP) resistance, the transcription profile analysis was performed and a RNA regulatory model of CC DDP resistance was proposed.

Methods: In this study, whole-transcriptome sequencing analysis was conducted to study the ncRNA and mRNA profiles of parental SiHa cells and DDP resistant SiHa/DDP cells. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were performed for pathway analysis based on the selected genes with significant differences in expression. Subsequently, ceRNA network analyses were conducted using the drug resistance-related genes and signal-transduction pathways by Cytoscape software. Furthermore, a ceRNA regulatory pathway, namely lncRNA-AC010198.2/hsa-miR-34b-3p/STC2, was selected by RT-qPCR validation and literature searching. Further validation was done by both dual-luciferase reporter gene assays and RNA pull-down assays. Besides that, the changes in gene expression and biological function were further studied by performing si-AC010198.2 transfection and DDP resistance analyses in the SiHa and SiHa/DDP cells, respectively.

Results: Using bioinformatics and dual-luciferase reporter gene analyses, we found that AC010198.2/miR-34b-3p/STC2 may be a key pathway for DDP resistance in CC cells. Significant differences in both downstream gene expression and the biological function assays including colony formation, migration efficiency and cell apoptosis were identified in AC010198.2 knockdown cells.

Conclusions: Our study will not only provide new markers and potential mechanism models for CC DDP resistance, but also discover novel targets for attenuating it.

Citing Articles

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miR-34b-3p-mediated regulation of STC2 and FN1 enhances chemosensitivity and inhibits proliferation in cervical cancer.

Jin S, Wang W, Xu X, Yu Z, Feng Z, Xie J Acta Biochim Biophys Sin (Shanghai). 2024; 56(5):740-752.

PMID: 38477044 PMC: 11177115. DOI: 10.3724/abbs.2024009.

The Regulatory Functions and the Mechanisms of Long Non-Coding RNAs in Cervical Cancer.

Yang Q, Al-Hendy A Cells. 2022; 11(7).

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