Fishing of α-Glucosidase's Ligands from by α-Glucosidase Functionalized Magnetic Nanoparticles

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2021 Oct 13

PMID 34641385

Authors

Hao Yuan

Hong Wan

Yi-Kao Hu

Emmanuel Ayodeji Ayeni

Qiang Chang

Chao Ma

Xun Liao

Affiliations

Soon will be listed here.

Abstract

α-Glucosidase was immobilized on magnetic nanoparticles (MNPs) for selective solid-phase extraction of the enzyme's ligands present in , which is a medicinal plant used for the treatment of various diseases and possesses anti-diabetic activity. One new compound, aloeacone (), together with two known compounds, aloenin aglycone () and aloin A (), were fished out as the enzyme's ligands. The structure of was determined by HR-MS and comprehensive NMR techniques. Compound exhibited a weak inhibitory effect on α-glucosidase, while compounds and were found to possess activation effects on the enzyme for the first time. It is interesting that both an inhibitor and agonists of α-glucosidase were fished out in one experiment.

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