Rapid Diagnostic Methods for SARS-CoV-2 (COVID-19) Detection: an Evidence-based Report

Overview

Journal J Med Life

Specialty General Medicine

Date 2021 Oct 8

PMID 34621365

Citations 4

Authors

Manish Kumar Verma

Parshant Kumar Sharma

Henu Kumar Verma

Anand Narayan Singh

Desh Deepak Singh

Poonam Verma

Areena Hoda Siddiqui

Affiliations

Soon will be listed here.

Abstract

Since December 2019, the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has been a global health concern. The transmission method is human-to-human. Since this second wave of SARS-CoV-2 is more aggressive than the first wave, rapid testing is warranted to use practical diagnostics to break the transfer chain. Currently, various techniques are used to diagnose SARS-CoV-2 infection, each with its own set of advantages and disadvantages. A full review of online databases such as PubMed, EMBASE, Web of Science, and Google Scholar was analyzed to identify relevant articles focusing on SARS-CoV-2 and diagnosis and therapeutics. The most recent article search was on May 10, 2021. We summarize promising methods for detecting the novel Coronavirus using sensor-based diagnostic technologies that are sensitive, cost-effective, and simple to use at the point of care. This includes loop-mediated isothermal amplification and several laboratory protocols for confirming suspected 2019-nCoV cases, as well as studies with non-commercial laboratory protocols based on real-time reverse transcription-polymerase chain reaction and a field-effect transistor-based bio-sensing device. We discuss a potential discovery that could lead to the mass and targeted SARS-CoV-2 detection needed to manage the COVID-19 pandemic through infection succession and timely therapy.

Citing Articles

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References

Jiang F, Deng L, Zhang L, Cai Y, Cheung C, Xia Z . Review of the Clinical Characteristics of Coronavirus Disease 2019 (COVID-19). J Gen Intern Med. 2020; 35(5):1545-1549. PMC: 7088708. DOI: 10.1007/s11606-020-05762-w. View

Chung M, Bernheim A, Mei X, Zhang N, Huang M, Zeng X . CT Imaging Features of 2019 Novel Coronavirus (2019-nCoV). Radiology. 2020; 295(1):202-207. PMC: 7194022. DOI: 10.1148/radiol.2020200230. View

Harbourt D, Haddow A, Piper A, Bloomfield H, Kearney B, Fetterer D . Modeling the stability of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) on skin, currency, and clothing. PLoS Negl Trop Dis. 2020; 14(11):e0008831. PMC: 7676723. DOI: 10.1371/journal.pntd.0008831. View

Ouassou H, Kharchoufa L, Bouhrim M, Daoudi N, Imtara H, Bencheikh N . The Pathogenesis of Coronavirus Disease 2019 (COVID-19): Evaluation and Prevention. J Immunol Res. 2020; 2020:1357983. PMC: 7352127. DOI: 10.1155/2020/1357983. View

Chotiwan N, Brewster C, Magalhaes T, Weger-Lucarelli J, Duggal N, Ruckert C . Rapid and specific detection of Asian- and African-lineage Zika viruses. Sci Transl Med. 2017; 9(388). PMC: 5654541. DOI: 10.1126/scitranslmed.aag0538. View

Li Z, Yi Y, Luo X, Xiong N, Liu Y, Li S . Development and clinical application of a rapid IgM-IgG combined antibody test for SARS-CoV-2 infection diagnosis. J Med Virol. 2020; 92(9):1518-1524. PMC: 7228300. DOI: 10.1002/jmv.25727. View

Chu D, Pan Y, Cheng S, Hui K, Krishnan P, Liu Y . Molecular Diagnosis of a Novel Coronavirus (2019-nCoV) Causing an Outbreak of Pneumonia. Clin Chem. 2020; 66(4):549-555. PMC: 7108203. DOI: 10.1093/clinchem/hvaa029. View

Kaarj K, Akarapipad P, Yoon J . Simpler, Faster, and Sensitive Zika Virus Assay Using Smartphone Detection of Loop-mediated Isothermal Amplification on Paper Microfluidic Chips. Sci Rep. 2018; 8(1):12438. PMC: 6102244. DOI: 10.1038/s41598-018-30797-9. View

Zhou P, Yang X, Wang X, Hu B, Zhang L, Zhang W . A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020; 579(7798):270-273. PMC: 7095418. DOI: 10.1038/s41586-020-2012-7. View

10.

Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N . Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 2000; 28(12):E63. PMC: 102748. DOI: 10.1093/nar/28.12.e63. View

11.

Wrapp D, Wang N, Corbett K, Goldsmith J, Hsieh C, Abiona O . Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation. Science. 2020; 367(6483):1260-1263. PMC: 7164637. DOI: 10.1126/science.abb2507. View

12.

Liu I, Chen P, Yeh S, Chiang Y, Huang L, Chang M . Immunofluorescence assay for detection of the nucleocapsid antigen of the severe acute respiratory syndrome (SARS)-associated coronavirus in cells derived from throat wash samples of patients with SARS. J Clin Microbiol. 2005; 43(5):2444-8. PMC: 1153760. DOI: 10.1128/JCM.43.5.2444-2448.2005. View

13.

Dai W, Zhang H, Yu J, Xu H, Chen H, Luo S . CT Imaging and Differential Diagnosis of COVID-19. Can Assoc Radiol J. 2020; 71(2):195-200. PMC: 7140975. DOI: 10.1177/0846537120913033. View

14.

Lau S, Woo P, Wong B, Tsoi H, Woo G, Poon R . Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in sars patients by enzyme-linked immunosorbent assay. J Clin Microbiol. 2004; 42(7):2884-9. PMC: 446266. DOI: 10.1128/JCM.42.7.2884-2889.2004. View

15.

Singh D, Han I, Choi E, Yadav D . Immunopathology, host-virus genome interactions, and effective vaccine development in SARS-CoV-2. Comput Struct Biotechnol J. 2020; 18:3774-3787. PMC: 7677077. DOI: 10.1016/j.csbj.2020.11.011. View

16.

Chen Y, Chan K, Hong C, Kang Y, Ge S, Chen H . A highly specific rapid antigen detection assay for on-site diagnosis of MERS. J Infect. 2016; 73(1):82-4. PMC: 7127149. DOI: 10.1016/j.jinf.2016.04.014. View

17.

Wang Z, Chen Y, Yang J, Han Y, Shi J, Zhan S . External Quality Assessment for Molecular Detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in Clinical Laboratories. J Mol Diagn. 2020; 23(1):19-28. PMC: 7587075. DOI: 10.1016/j.jmoldx.2020.10.008. View

18.

Corman V, Landt O, Kaiser M, Molenkamp R, Meijer A, Chu D . Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Euro Surveill. 2020; 25(3). PMC: 6988269. DOI: 10.2807/1560-7917.ES.2020.25.3.2000045. View

19.

La Marca A, Capuzzo M, Paglia T, Roli L, Trenti T, Nelson S . Testing for SARS-CoV-2 (COVID-19): a systematic review and clinical guide to molecular and serological in-vitro diagnostic assays. Reprod Biomed Online. 2020; 41(3):483-499. PMC: 7293848. DOI: 10.1016/j.rbmo.2020.06.001. View

20.

Wang D, Hu B, Hu C, Zhu F, Liu X, Zhang J . Clinical Characteristics of 138 Hospitalized Patients With 2019 Novel Coronavirus-Infected Pneumonia in Wuhan, China. JAMA. 2020; 323(11):1061-1069. PMC: 7042881. DOI: 10.1001/jama.2020.1585. View