The Chloroplast Ribosomal Protein Large Subunit 1 Interacts with Viral Polymerase and Promotes Virus Infection

Overview

Journal Plant Physiol

Specialty Physiology

Date 2021 Oct 7

PMID 34618134

Citations 12

Authors

De-Jie Cheng

Xiao-Jie Xu

Zhi-Yong Yan

Carlos Kwesi Tettey

Le Fang

Guang-Ling Yang

Chao Geng

Yan-Ping Tian

Xiang-Dong Li

Affiliations

Soon will be listed here.

Abstract

Chloroplasts play an indispensable role in the arms race between plant viruses and hosts. Chloroplast proteins are often recruited by plant viruses to support viral replication and movement. However, the mechanism by which chloroplast proteins regulate potyvirus infection remains largely unknown. In this study, we observed that Nicotiana benthamiana ribosomal protein large subunit 1 (NbRPL1), a chloroplast ribosomal protein, localized to the chloroplasts via its N-terminal 61 amino acids (transit peptide), and interacted with tobacco vein banding mosaic virus (TVBMV) nuclear inclusion protein b (NIb), an RNA-dependent RNA polymerase. Upon TVBMV infection, NbRPL1 was recruited into the 6K2-induced viral replication complexes in chloroplasts. Silencing of NbRPL1 expression reduced TVBMV replication. NbRPL1 competed with NbBeclin1 to bind NIb, and reduced the NbBeclin1-mediated degradation of NIb. Therefore, our results suggest that NbRPL1 interacts with NIb in the chloroplasts, reduces NbBeclin1-mediated NIb degradation, and enhances TVBMV infection.

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