Mass-sensitive Particle Tracking to Elucidate the Membrane-associated MinDE Reaction Cycle

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2021 Oct 5

PMID 34608318

Citations 25

Authors

Tamara Heermann

Frederik Steiert

Beatrice Ramm

Nikolas Hundt

Petra Schwille

Affiliations

Soon will be listed here.

Abstract

In spite of their great importance in biology, methods providing access to spontaneous molecular interactions with and on biological membranes have been sparse. The recent advent of mass photometry to quantify mass distributions of unlabeled biomolecules landing on surfaces raised hopes that this approach could be transferred to membranes. Here, by introducing a new interferometric scattering (iSCAT) image processing and analysis strategy adapted to diffusing particles, we enable mass-sensitive particle tracking (MSPT) of single unlabeled biomolecules on a supported lipid bilayer. We applied this approach to the highly nonlinear reaction cycles underlying MinDE protein self-organization. MSPT allowed us to determine the stoichiometry and turnover of individual membrane-bound MinD/MinDE protein complexes and to quantify their size-dependent diffusion. This study demonstrates the potential of MSPT to enhance our quantitative understanding of membrane-associated biological systems.

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