Study the Antioxidant Effects of Blue-green Algae Spirulina Extract on ROS and MDA Production in Human Lung Cancer Cells

Overview

Journal Biochem Biophys Rep

Specialty Biochemistry

Date 2021 Oct 4

PMID 34604549

Citations 7

Authors

Elham Tajvidi

Nikta Nahavandizadeh

Maryam Pournaderi

Azin Zargar Pourrashid

Fatemeh Bossaghzadeh

Zahra Khoshnood

Affiliations

Soon will be listed here.

Abstract

In order to study the effects of Spirulina, , two cell lines of A549 and HFF were treated with the concentration of IC50 for 24 h. MTT analysis showed that the highest decrease in viability of cells happened at the concentration of 500 μg/ml. The necrosis, releases of LDH, produced DCFH, and Lipid peroxidation were higher in the cancer cell lines in comparison to normal cells. Results showed that the extract affected the cell cycle of the A549 cell line. Also, the algal extract had concentration-dependent antioxidant activity. Also, the production of malonyl dialdehyde was significantly higher in treated cells and there was a significant relationship between produced MDA and ROS. Results showed that extract had a remarkable effect on the lung cancer cell cycle and arrest the cell cycle in phase G; so the cells didn't enter phase M and the proliferation of cancer cells prevented. Furthermore, according to the higher production of ROS and MDA in treated A549 cancer cell lines, it could be concluded that this algal extract could be considered as a natural product with anticancer activity against lung cancer cells.

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