The Off-Targets of Clustered Regularly Interspaced Short Palindromic Repeats Gene Editing

Overview

Journal Front Cell Dev Biol

Specialty Cell Biology

Date 2021 Oct 4

PMID 34604217

Citations 10

Authors

Manuel M Vicente

Miguel Chaves-Ferreira

Joao M P Jorge

Joao T Proenca

Vasco M Barreto

Affiliations

Soon will be listed here.

Abstract

The repurposing of the CRISPR/Cas bacterial defense system against bacteriophages as simple and flexible molecular tools has revolutionized the field of gene editing. These tools are now widely used in basic research and clinical trials involving human somatic cells. However, a global moratorium on all clinical uses of human germline editing has been proposed because the technology still lacks the required efficacy and safety. Here we focus on the approaches developed since 2013 to decrease the frequency of unwanted mutations (the off-targets) during CRISPR-based gene editing.

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