Functional Investigation of AfsKRS Regulatory System for Pristinamycin Biosynthesis in

Overview

Journal 3 Biotech

Publisher Springer

Specialty Biotechnology

Date 2021 Oct 4

PMID 34603918

Citations 1

Authors

Qingchao Jin

Haipeng Liao

Yanping Dou

Na Shen

Zhige Wu

Yu Yang

Zhihua Jin

Affiliations

Soon will be listed here.

Abstract

Three genes encoding AfsK, AfsR, AfsS homologues in were studied, respectively, to investigate regulatory role of AfsKRS system for pristinamycin biosynthesis. Transcription change and gene inactivation analysis indicated that these genes had active transcription and positive regulation for the improvement of pristinamycin production in . The analysis of AfsKRS-defective mutagenesis indicated that there might be a positive correlation between the product of and pristinamycin I biosynthesis, and a negative correlation to pristinamycin II biosynthesis. However, both and might have negative correlation to pristinamycin I production and positive correlation to pristinamycin II production. The effects on pristinamycin production of AfsKRS disruptants by protein kinase inhibitor K252a indicated that AfsR, both not AfsK and AfsS, was the inhibition target of K252a in , and AfsR should serve as a pleiotropic regulator to have differential regulation on biosynthesis of pristinamycin I and II components. Based on above study, it might be deduced that different signal transduction patterns via AfsK, AfsR, AfsS of AfsKRS system should be involved in respective regulation for biosynthesis of pristinamycin I and II in . In conclusion, the investigation could give some valuable clues for exploring furtherly regulatory function of AfsKRS system in .

Citing Articles

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Yan Y, Xia H Front Microbiol. 2024; 15:1368809.

PMID: 38550856 PMC: 10972967. DOI: 10.3389/fmicb.2024.1368809.

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