Identification and Analysis of Gene Family Indicated That Promotes Plant Growth and Development Through Brassinosteroid Signaling in Cotton ( L.)

Overview

Journal Front Plant Sci

Date 2021 Oct 4

PMID 34603349

Citations 3

Authors

Shengdong Li

Zhao Liu

Guoquan Chen

Ghulam Qanmber

LiLi Lu

Jiaxin Zhang

Shuya Ma

Zuoren Yang

Fuguang Li

Affiliations

Soon will be listed here.

Abstract

Brassinosteroids (BRs), an efficient plant endogenous hormone, significantly promotes plant nutrient growth adapting to biological and abiotic adversities. BRs mainly promote plant cell elongation by regulating gene expression patterns. () genes have been characterized as the indicators of BR response genes. Cotton, an ancient crop, is of great economic value and its fibers can be made into all kinds of fabrics. However, gene family genes have not been full identified in cotton. 175 genes were identified in nine plant species, of which 39 genes in in our study. A phylogenetic analysis grouped all of the proteins encoded by the genes into five major clades. Sequence identification of conserved amino acid residues among monocotyledonous and dicotyledonous species showed a high level of conservation across the N and C terminal regions. Only 25% the genes contain introns besides conserved gene structure and protein motifs distribution. The 39 genes were unevenly distributed on the 18 At and Dt sub-genome chromosomes. Most of the genes were derived from gene duplication events, while only three genes showed evidence of tandem duplication. Homologous locus relationships showed that 15 genes are located on collinear blocks and that all orthologous/paralogous gene pairs had > values, indicating purifying selection pressure. The genes showed ubiquitous expression in all eight tested cotton tissues and following exposure to three phytohormones, IAA, GA, and BL. Furthermore, was mainly expressed in response to BL treatment, and was predominantly expressed in the fibers. was found to be a plasma membrane protein, and its ectopic expression in mediated BR-regulated plant growth and development with altered expression of , , , and . Additionally, the functions of were confirmed by virus-induced gene silencing (VIGS) in cotton. This study will provide important genetic resources for future cotton breeding programs.

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