Leukemogenesis Model Using Retrovirus Transduction

Overview

Journal Bio Protoc

Specialty Biology

Date 2021 Oct 1

PMID 34595295

Citations 5

Authors

Hiroshi Okuda

Akihiko Yokoyama

Affiliations

Soon will be listed here.

Abstract

Various genetic alterations such as chromosomal translocation cause leukemia. For examples, gene rearrangements of the mixed-lineage leukemia () gene generate fusion genes, whose products are potent oncogenic drivers in acute leukemia. To better understand the mechanism of disease onset, several murine leukemia models using retroviral gene transduction, xenograft, or Cre-mediated chromosomal translocation have been developed over the past twenty years. Particularly, a retroviral gene transduction-mediated murine leukemia model has been frequently used in the leukemia research field. Here, we describe the detailed protocol for this model.

Citing Articles

MOZ/ENL complex is a recruiting factor of leukemic AF10 fusion proteins.

Komata Y, Kanai A, Maeda T, Inaba T, Yokoyama A Nat Commun. 2023; 14(1):1979.

PMID: 37031220 PMC: 10082848. DOI: 10.1038/s41467-023-37712-5.

RNA-binding proteins of KHDRBS and IGF2BP families control the oncogenic activity of MLL-AF4.

Okuda H, Miyamoto R, Takahashi S, Kawamura T, Ichikawa J, Harada I Nat Commun. 2022; 13(1):6688.

PMID: 36335100 PMC: 9637093. DOI: 10.1038/s41467-022-34558-1.

HBO1-MLL interaction promotes AF4/ENL/P-TEFb-mediated leukemogenesis.

Takahashi S, Kanai A, Okuda H, Miyamoto R, Komata Y, Kawamura T Elife. 2021; 10.

PMID: 34431785 PMC: 8387021. DOI: 10.7554/eLife.65872.

HOXA9 promotes MYC-mediated leukemogenesis by maintaining gene expression for multiple anti-apoptotic pathways.

Miyamoto R, Kanai A, Okuda H, Komata Y, Takahashi S, Matsui H Elife. 2021; 10.

PMID: 34310280 PMC: 8313233. DOI: 10.7554/eLife.64148.

Spontaneous and Induced Animal Models for Cancer Research.

Onaciu A, Munteanu R, Munteanu V, Gulei D, Raduly L, Feder R Diagnostics (Basel). 2020; 10(9).

PMID: 32878340 PMC: 7555044. DOI: 10.3390/diagnostics10090660.

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