Super-Resolution Microscopy: Shedding New Light on Imaging

Overview

Journal Front Chem

Specialty Chemistry

Date 2021 Oct 1

PMID 34595156

Citations 13

Authors

Yingying Jing

Chenshuang Zhang

Bin Yu

Danying Lin

Junle Qu

Affiliations

Soon will be listed here.

Abstract

Over the past two decades, super-resolution microscopy (SRM), which offered a significant improvement in resolution over conventional light microscopy, has become a powerful tool to visualize biological activities in both fixed and living cells. However, completely understanding biological processes requires studying cells in a physiological context at high spatiotemporal resolution. Recently, SRM has showcased its ability to observe the detailed structures and dynamics in living species. Here we summarized recent technical advancements in SRM that have been successfully applied to imaging. Then, improvements in the labeling strategies are discussed together with the spectroscopic and chemical demands of the fluorophores. Finally, we broadly reviewed the current applications for super-resolution techniques in living species and highlighted some inherent challenges faced in this emerging field. We hope that this review could serve as an ideal reference for researchers as well as beginners in the relevant field of super resolution imaging.

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