Loquacious-PD Regulates the Terminus-dependent Molecular Recognition of Dicer-2 Toward Double-stranded RNA

Overview

Journal Chem Commun (Camb)

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2021 Sep 30

PMID 34590626

Citations 2

Authors

McKenzie Jonely

Raushan K Singh

Helen M Donelick

Brenda L Bass

Rodrigo Noriega

Affiliations

Soon will be listed here.

Abstract

Dicer-2 cleaves double-stranded RNA into siRNAs in a terminus-dependent manner as part of 's RNA interference pathway. Using ultrafast fluorescence, we probe the local environment of chromophores at the dsRNA terminus upon binding by Dicer-2 and interrogate the effects of Loquacious-PD, an accessory protein. We find substrate-selective modes of molecular recognition that distinguish between blunt and 3'overhang termini, but whose differences are greatly reduced by Loquacious-PD. These results connect the molecular recognition properties of Dicer-2 to its selective processing of dsRNAs with different termini and to its need for Loquacious-PD to efficiently produce endogenous siRNAs.

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