Elastic Property of Sickle Cell Anemia and Sickle Cell Trait Red Blood Cells

Overview

Journal J Biomed Opt

Specialties Biomedical Engineering
Ophthalmology

Date 2021 Sep 30

PMID 34590447

Citations 4

Authors

Endris Muhammed

James Cooper

Daniel Devito

Robert Mushi

Maria Del Pilar Aguinaga

Daniel Erenso

Horace Crogman

Affiliations

Soon will be listed here.

Abstract

Significance: We introduce a model for better calibration of the trapping force using an equal but oppositely directed drag force acting on a trapped red blood cell (RBC). We demonstrate this approach by studying RBCs' elastic properties from deidentified sickle cell anemia (SCA) and sickle cell trait (SCT) blood samples.

Aim: A laser trapping (LT) force was formulated and analytically calculated in a cylindrical model. Using this trapping force relative percent difference, the maximum (longitudinal) and minimum (transverse) radius rate and stiffness were used to study the elasticity.

Approach: The elastic property of SCA and SCT RBCs was analyzed using LT technique with computer controlled piezo-driven stage, in order to trap and stretch the RBCs.

Results: For all parameters, the results show that the SCT RBC samples have higher elastic property than the SCA RBCs. The higher rigidity in the SCA cell may be due to the lipid composition of the membrane, which was affected by the cholesterol concentration.

Conclusions: By developing a theoretical model for different trapping forces, we have also studied the elasticity of RBCs in SCT (with hemoglobin type HbAS) and in SCA (with hemoglobin type HbSS). The results for the quantities describing the elasticity of the cells consistently showed that the RBCs in the SCT display lower rigidity and higher deformability than the RBCs with SCA.

Citing Articles

Cardiovascular consequences of sickle cell disease.

Palomarez A, Jha M, Medina Romero X, Horton R Biophys Rev (Melville). 2024; 3(3):031302.

PMID: 38505276 PMC: 10903381. DOI: 10.1063/5.0094650.

Observation of magnet-induced star-like radiation of a plasma created from cancer cells in a laser trap.

Erenso D, Tran L, Abualrob I, Bushra M, Hengstenberg J, Muhammed E Eur Biophys J. 2024; 53(3):123-131.

PMID: 38451329 DOI: 10.1007/s00249-024-01701-3.

Measurement of Charge and Refractive Indices in Optically Trapped and Ionized Living Cells.

Muhammed E, Erenso D, Gao Y, Chen L, Kelley M, Vazquez C Tomography. 2023; 9(1):70-88.

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The radiation response measurement of a single and multiple cell ionization of neuroblastoma cells by infrared laser trap.

Goangul M, Stewart W, Erenso D, Crogman H J Radiat Res. 2022; 64(1):113-125.

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