Comparative Analysis of Biofilm Formation by Candida Albicans and Candida Krusei in Different Types of Contact Lenses

Overview

Journal Arq Bras Oftalmol

Specialty Ophthalmology

Date 2021 Sep 29

PMID 34586234

Citations 2

Authors

Laura Nagy Fritsch

Amanda Latercia Tranches Dias

Naiara Chaves Silva

Geraldo Jose Medeiros Fernandes

Flavia Beatriz de Andrade Oliveira Ribeiro

Affiliations

Soon will be listed here.

Abstract

Objective: To evaluate the Candida krusei and Candida albicans biofilm formation abilities on 5 different types of contact lenses and compare their metabolic activities and biomass.

Methods: After biofilm formation by both the test species, their metabolic activity was assessed by the 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide reduction assay with menadione, while the biomass was determined by staining with 0.4% crystal violet dye for further statistical analysis.

Results: Both the Candida species could form biofilms on different types of contact lenses, with greater metabolic activities and lower biomass formation in rigid gas permeable lenses.

Conclusion: Biofilm formation with greater metabolic activity and greater biomass were expected on soft contact lenses considering their surface hydrophobicity. However, the results demonstrated a greater metabolic activity on rigid contact lenses. This result has a great significance with regards to the increasing risk of microbial keratitis, although further studies are warranted to better elucidate the formation of biofilms on different types of contact lens materials in the future.

Citing Articles

The Role of Biofilms in Contact Lens Associated Fungal Keratitis.

Yi J, Sun Y, Zeng C, Kostoulias X, Qu Y Antibiotics (Basel). 2023; 12(10).

PMID: 37887234 PMC: 10604847. DOI: 10.3390/antibiotics12101533.

Candida Biofilm Eye Infection: Main Aspects and Advance in Novel Agents as Potential Source of Treatment.

Petrillo F, Sinoca M, Fea A, Galdiero M, Maione A, Galdiero E Antibiotics (Basel). 2023; 12(8).

PMID: 37627697 PMC: 10451181. DOI: 10.3390/antibiotics12081277.

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