Screening Paracoccidioidomycosis by Double Immunodiffusion Test in a Referred Diagnostic Center in Brazilian Southeastern: an Accessible Tool

Overview

Journal Infection

Date 2021 Sep 28

PMID 34580797

Citations 4

Authors

Simone Bravim Maifrede

Wdson Luis Lima Kruschewsky

Suzana Alves Patricio

Aloisio Falqueto

Paulo Mendes Pecanha

Luiz Cosme Cotta Malaquias

Ana Paula Possa

Zoilo Pires De Camargo

Anderson Messias Rodrigues

Sarah Santos Goncalves

Tania Regina Grao-Velloso

Affiliations

Soon will be listed here.

Abstract

Purpose: This article shows reports of the clinical-epidemiological characteristics and serological screening in patients assisted by a reference center for PCM care, University Hospital Cassiano Antonio Moraes, Federal University of Espirito Santo, Brazil.

Methods: The patient's sera with PCM were analyzed by DID test at the beginning and the end treatment. Clinical and demographic data were also collected to characterize the sample.

Results: One hundred patients with a suspected diagnosis of PCM were evaluated. Serology by DID test was used as a screen in all patients. The test was positive for 79 patients (72 for Paracoccidioides brasiliensis and 7 for Paracoccidioides lutzii). Serology was negative in 21 sera, although all of them were diagnosed PCM by histopathologic or direct exam. Serological follow-up was performed during the treatment of all patients. After treatment, 58(58%) had negative serology and 33(33%) low levels of antibodies (≤ 1:16).

Conclusion: Our results indicate the importance of the DID test for the screening and monitoring of PCM and that the incidence of P. lutzii might be greater than expected in areas where it is not the predominant PCM species. Therefore, this article may contribute to improving the knowledge and clinical management about this disease.

Citing Articles

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Development of a Multiplex qPCR Assay for Fast Detection and Differentiation of Paracoccidioidomycosis Agents.

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