Hyaluronic Acid/Collagen Nanofiber Tubular Scaffolds Support Endothelial Cell Proliferation, Phenotypic Shape and Endothelialization

Overview

Journal Nanomaterials (Basel)

Date 2021 Sep 28

PMID 34578649

Citations 11

Authors

Yuqing Niu

Massimiliano Galluzzi

Affiliations

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Abstract

In this study, we designed and synthetized artificial vascular scaffolds based on nanofibers of collagen functionalized with hyaluronic acid (HA) in order to direct the phenotypic shape, proliferation, and complete endothelization of mouse primary aortic endothelial cells (PAECs). Layered tubular HA/collagen nanofibers were prepared using electrospinning and crosslinking process. The obtained scaffold is composed of a thin inner layer and a thick outer layer that structurally mimic the layer the intima and media layers of the native blood vessels, respectively. Compared with the pure tubular collagen nanofibers, the surface of HA functionalized collagen nanofibers has higher anisotropic wettability and mechanical flexibility. HA/collagen nanofibers can significantly promote the elongation, proliferation and phenotypic shape expression of PAECs. In vitro co-culture of mouse PAECs and their corresponding smooth muscle cells (SMCs) showed that the luminal endothelialization governs the biophysical integrity of the newly formed extracellular matrix (e.g., collagen and elastin fibers) and structural remodeling of SMCs. Furthermore, in vitro hemocompatibility assays indicated that HA/collagen nanofibers have no detectable degree of hemolysis and coagulation, suggesting their promise as engineered vascular implants.

Citing Articles

Moving Toward Biomimetic Tissue-Engineered Scaffolds.

Baiguera S, Di Silvio L, Del Gaudio C Nanomaterials (Basel). 2024; 14(24).

PMID: 39728564 PMC: 11677525. DOI: 10.3390/nano14242028.

Investigating the efficacy of uncrosslinked porcine collagen coated vascular grafts for neointima formation and endothelialization.

Yang C, Su C, Zou J, Zhong B, Wang L, Chen B Front Bioeng Biotechnol. 2024; 12:1418259.

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Correction: Niu, Y.; Galluzzi, M. Hyaluronic Acid/Collagen Nanofiber Tubular Scaffolds Support Endothelial Cell Proliferation, Phenotypic Shape and Endothelialization. 2021, , 2334.

Niu Y, Galluzzi M Nanomaterials (Basel). 2024; 14(14).

PMID: 39057912 PMC: 11280418. DOI: 10.3390/nano14141203.

Development of Biocompatible 3D-Printed Artificial Blood Vessels through Multidimensional Approaches.

Choi J, Lee E, Jang W, Kwon S J Funct Biomater. 2023; 14(10).

PMID: 37888162 PMC: 10607080. DOI: 10.3390/jfb14100497.

Nanofiber Scaffolds as Drug Delivery Systems Promoting Wound Healing.

Jiang Z, Zheng Z, Yu S, Gao Y, Ma J, Huang L Pharmaceutics. 2023; 15(7).

PMID: 37514015 PMC: 10384736. DOI: 10.3390/pharmaceutics15071829.

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