ITS2 Sequencing and Targeted Meta-Proteomics of Infant Gut Mycobiome Reveal the Functional Role of Sp. During Atopic Dermatitis Manifestation

Overview

Journal J Fungi (Basel)

Date 2021 Sep 28

PMID 34575786

Citations 11

Authors

Kevin Mok

Narissara Suratanon

Sittiruk Roytrakul

Sawanya Charoenlappanit

Preecha Patumcharoenpol

Pantipa Chatchatee

Wanwipa Vongsangnak

Massalin Nakphaichit

Affiliations

Soon will be listed here.

Abstract

Association between the gut mycobiome and atopic dermatitis was investigated in 9-12-month-old infants using metagenomics. Two groups of atopic dermatitis infants were classified according to their symptom development as outgrown (recovered) and persisted (still undergoing). The evenness and diversity of the mycobiome in the persisted group were higher than in the healthy and outgrown groups. Dysbiosis of the microbiome in the persisted group was observed by a reduction in the / ratio. Five fungi were selected as markers from each sample group. In the persisted group, sp. abundance increased significantly, while sp. and sp. abundance increased in the healthy group, and sp. and sp. abundance increased considerably in the outgrown group. Metaproteomic analysis revealed that the persisted group had a high abundance of fungal proteins, particularly those from sp. Unique proteins such as RAN-binding protein 1 and glycerol kinase from sp. were hypothesized to be related to atopic dermatitis manifestation in infants.

Citing Articles

Impact of diet on the gut mycobiome of Hong Kong Chinese infants.

Fong J, Lok K, Yeung M, Li W, Woo P, Teng J Comput Struct Biotechnol J. 2025; 27:661-671.

PMID: 40061440 PMC: 11889518. DOI: 10.1016/j.csbj.2025.02.006.

Exploring Protein Functions of Gut Bacteriome and Mycobiome in Thai Infants Associated with Atopic Dermatitis Through Metaproteomic and Host Interaction Analysis.

Chantanaskul T, Patumcharoenpol P, Roytrakul S, Kingkaw A, Vongsangnak W Int J Mol Sci. 2025; 25(24.

PMID: 39769296 PMC: 11676981. DOI: 10.3390/ijms252413533.

Gut microbiota profile and atopic dermatitis in the first year of life.

Pantazi A, Nori W, Kassim M, Balasa A, Mihai C, Chisnoiu T J Med Life. 2024; 17(10):948-952.

PMID: 39720170 PMC: 11665751. DOI: 10.25122/jml-2024-0287.

Gut Mycobiome in Atopic Dermatitis and in Overweight Young Children: A Prospective Cohort Study in Finland.

Vanni P, Turunen J, Aijala V, Tapiainen V, Paalanne M, Pokka T J Fungi (Basel). 2024; 10(5).

PMID: 38786688 PMC: 11122208. DOI: 10.3390/jof10050333.

Preliminary characterization of gut mycobiome enterotypes reveals the correlation trends between host metabolic parameter and diet: a case study in the Thai Cohort.

Mok K, Poolsawat T, Somnuk S, Wanikorn B, Patumcharoenpol P, Nitisinprasert S Sci Rep. 2024; 14(1):5805.

PMID: 38461361 PMC: 10924899. DOI: 10.1038/s41598-024-56585-2.

References

Sokol H, Leducq V, Aschard H, Pham H, Jegou S, Landman C . Fungal microbiota dysbiosis in IBD. Gut. 2016; 66(6):1039-1048. PMC: 5532459. DOI: 10.1136/gutjnl-2015-310746. View

Song H, Yoo Y, Hwang J, Na Y, Kim H . Faecalibacterium prausnitzii subspecies-level dysbiosis in the human gut microbiome underlying atopic dermatitis. J Allergy Clin Immunol. 2015; 137(3):852-60. DOI: 10.1016/j.jaci.2015.08.021. View

Lemoinne S, Kemgang A, Belkacem K, Straube M, Jegou S, Corpechot C . Fungi participate in the dysbiosis of gut microbiota in patients with primary sclerosing cholangitis. Gut. 2019; 69(1):92-102. DOI: 10.1136/gutjnl-2018-317791. View

Chiaro T, Soto R, Stephens W, Kubinak J, Petersen C, Gogokhia L . A member of the gut mycobiota modulates host purine metabolism exacerbating colitis in mice. Sci Transl Med. 2017; 9(380). PMC: 5994919. DOI: 10.1126/scitranslmed.aaf9044. View

Tuon F, Costa S . Rhodotorula infection. A systematic review of 128 cases from literature. Rev Iberoam Micol. 2008; 25(3):135-40. DOI: 10.1016/s1130-1406(08)70032-9. View

Edgar R . MUSCLE: a multiple sequence alignment method with reduced time and space complexity. BMC Bioinformatics. 2004; 5:113. PMC: 517706. DOI: 10.1186/1471-2105-5-113. View

Lynch S, Pedersen O . The Human Intestinal Microbiome in Health and Disease. N Engl J Med. 2016; 375(24):2369-2379. DOI: 10.1056/NEJMra1600266. View

. Structure, function and diversity of the healthy human microbiome. Nature. 2012; 486(7402):207-14. PMC: 3564958. DOI: 10.1038/nature11234. View

Nash A, Auchtung T, Wong M, Smith D, Gesell J, Ross M . The gut mycobiome of the Human Microbiome Project healthy cohort. Microbiome. 2017; 5(1):153. PMC: 5702186. DOI: 10.1186/s40168-017-0373-4. View

10.

Losuwannarak N, Maiuthed A, Kitkumthorn N, Leelahavanichkul A, Roytrakul S, Chanvorachote P . Gigantol Targets Cancer Stem Cells and Destabilizes Tumors via the Suppression of the PI3K/AKT and JAK/STAT Pathways in Ectopic Lung Cancer Xenografts. Cancers (Basel). 2019; 11(12). PMC: 6966687. DOI: 10.3390/cancers11122032. View

11.

Goravey W, Ali G, Abid F, Ibrahim E, Al Maslamani M, Hadi H . Central line-associated fungemia in an immunocompetent host: Case report and review of the literature. Clin Case Rep. 2021; 9(4):2158-2161. PMC: 8077285. DOI: 10.1002/ccr3.3969. View

12.

Mims T, Al Abdallah Q, Stewart J, Watts S, White C, Rousselle T . The gut mycobiome of healthy mice is shaped by the environment and correlates with metabolic outcomes in response to diet. Commun Biol. 2021; 4(1):281. PMC: 7935979. DOI: 10.1038/s42003-021-01820-z. View

13.

Lee S, Lee E, Park Y, Hong S . Microbiome in the Gut-Skin Axis in Atopic Dermatitis. Allergy Asthma Immunol Res. 2018; 10(4):354-362. PMC: 6021588. DOI: 10.4168/aair.2018.10.4.354. View

14.

Tsakok T, Marrs T, Mohsin M, Baron S, Du Toit G, Till S . Does atopic dermatitis cause food allergy? A systematic review. J Allergy Clin Immunol. 2016; 137(4):1071-1078. DOI: 10.1016/j.jaci.2015.10.049. View

15.

Gustafsson D, Sjoberg O, Foucard T . Development of allergies and asthma in infants and young children with atopic dermatitis--a prospective follow-up to 7 years of age. Allergy. 2001; 55(3):240-5. DOI: 10.1034/j.1398-9995.2000.00391.x. View

16.

Raethong N, Nakphaichit M, Suratannon N, Sathitkowitchai W, Weerapakorn W, Keawsompong S . Analysis of Human Gut Microbiome: Taxonomy and Metabolic Functions in Thai Adults. Genes (Basel). 2021; 12(3). PMC: 7996147. DOI: 10.3390/genes12030331. View

17.

Bardou P, Mariette J, Escudie F, Djemiel C, Klopp C . jvenn: an interactive Venn diagram viewer. BMC Bioinformatics. 2014; 15:293. PMC: 4261873. DOI: 10.1186/1471-2105-15-293. View

18.

Brunner P . Early immunologic changes during the onset of atopic dermatitis. Ann Allergy Asthma Immunol. 2019; 123(2):152-157. DOI: 10.1016/j.anai.2019.03.033. View

19.

Guttman-Yassky E, Diaz A, Pavel A, Fernandes M, Lefferdink R, Erickson T . Use of Tape Strips to Detect Immune and Barrier Abnormalities in the Skin of Children With Early-Onset Atopic Dermatitis. JAMA Dermatol. 2019; 155(12):1358-1370. PMC: 6802262. DOI: 10.1001/jamadermatol.2019.2983. View

20.

Eichenfield L, Ahluwalia J, Waldman A, Borok J, Udkoff J, Boguniewicz M . Current guidelines for the evaluation and management of atopic dermatitis: A comparison of the Joint Task Force Practice Parameter and American Academy of Dermatology guidelines. J Allergy Clin Immunol. 2017; 139(4S):S49-S57. DOI: 10.1016/j.jaci.2017.01.009. View