Iriflophenone-3-C-β-d Glucopyranoside from (Hope) C. Chr. with Promising Future As Natural Antibiotic for Gastrointestinal Tract Infections

Ethnopharmacological approaches provide clues for the search of bioactive compounds. (Hope) C. Chr. (plant family: Dryopteridaceae) is an ethnomedicinal plant of the Galliyat region of Pakistan. The aqueous fraction (AqF) of is being used by inhabitants of the Galliyat region of Pakistan to treat their gastrointestinal tract ailments, especially those caused by bacteria. The aims of the present study were as follows: (i) to justify the ethnomedicinal uses of the AqF of ; (ii) to isolate a bioactive compound from the AqF of ; and (iii) to evaluate the antibacterial and cytotoxic potential of the isolated compound. Column chromatography (CC) techniques were used for the isolation studies. Spectroscopic techniques (UV-Vis, MS, 1&2D NMR) were used for structural elucidation. The agar-well diffusion method was used to evaluate the antibacterial potential of "i3CβDGP" against five bacterial strains, and compare it with the known antibiotic "Cefixime". The brine shrimp lethality test (BSLT) was used for cytotoxic studies. The AqF of afforded "iriflophenone-3-C-β-D glucopyranoside (i3CβDGP)" when subjected to LH20 Sephadex, followed by MPLC silica gel, and purified by preparative TLC. The "i3CβDGP" showed a strong potential (MIC = 31.1 ± 7.2, 62.5 ± 7.2, and 62.5 ± 7.2 µg/mL) against , , and , respectively. On the other hand, the least antibacterial potential was shown by "i3CβDGP" (MIC = 125 ± 7.2 µg/mL), against , in comparison to Cefixime (MIC = 62.5 ± 7.2 µg/mL). The cytotoxicity of "i3CβDGP" was significantly low (LD = 10.037 ± 2.8 µg/mL) against nauplii. This study not only justified the ethnomedicinal use of , but also highlighted the importance of ethnomedicinal knowledge. Further studies on AqF and other fractions of are in progress.