Multifunctionality of Nanosized Calcium Apatite Dual-Doped with Li/Eu Ions Related to Cell Culture Studies and Cytotoxicity Evaluation In Vitro

Overview

Journal Biomolecules

Publisher MDPI

Specialties Biochemistry
Molecular Biology

Date 2021 Sep 28

PMID 34572601

Citations 3

Authors

Paulina Sobierajska

Blazej Pozniak

Marta Tikhomirov

Julia Miller

Lucyna Mrowczynska

Agata Piecuch

Justyna Rewak-Soroczynska

Agata Dorotkiewicz-Jach

Zuzanna Drulis-Kawa

Rafal J Wiglusz

Affiliations

Soon will be listed here.

Abstract

Li/Eu dual-doped calcium apatite analogues were fabricated using a microwave stimulated hydrothermal technique. XRPD, FT-IR, micro-Raman spectroscopy, TEM and SAED measurements indicated that obtained apatites are single-phased, crystallize with a hexagonal structure, have similar morphology and nanometric size as well as show red luminescence. Lithium effectively modifies the local symmetry of optical active sites and, thus, affects the emission efficiency. Moreover, the hydrodynamic size and surface charge of the nanoparticles have been extensively studied. The protein adsorption (lysozyme, LSZ; bovine serum albumin, BSA) on the nanoparticle surface depended on the type of cationic dopant (Li, Eu) and anionic group (OH, Cl, F) of the apatite matrix. Interaction with LSZ resulted in a positive zeta potential, and the nanoparticles had the lowest hydrodynamic size in this protein medium. The cytotoxicity assessment was carried out on the human osteosarcoma cell line (U2OS), murine macrophages (J774.E), as well as human red blood cells (RBCs). The studied apatites were not cytotoxic to RBCs and J774.E cells; however, at higher concentrations of nanoparticles, cytotoxicity was observed against the U2OS cell line. No antimicrobial activity was detected against Gram-negative bacteria with one exception for treated with Li-doped fluorapatite.

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