Effect of Insulin-like Growth Factor-1 Complex of Simmental Bull Seminal Plasma on Post-thawed Kacang Buck Semen Fertility

Overview

Journal Vet World

Specialty Veterinary Medicine

Date 2021 Sep 27

PMID 34566323

Citations 5

Authors

Suherni Susilowati

Imam Mustofa

Wurlina Wurlina

Indah Norma Triana

Suzanita Utama

Rimayanti Rimayanti

Affiliations

Soon will be listed here.

Abstract

Background And Aim: Kacang buck sperm is cryosensitive due to the seminal plasma of semen itself. Meanwhile, bull seminal plasma contains the insulin-like growth factor-1 (IGF-1) complex, which is cryoprotective. The addition of the crude protein of Simmental bull seminal plasma increased the quality of post-thawed semen of Kacang buck. The study was conducted to determine the effects of Simmental bull seminal plasma with IGF-1 on the fertility of post-thawed Kacang buck semen.

Materials And Methods: Buck semen was diluted in the following skim milk-egg yolk extender preparations: Without the addition of Simmental bull seminal plasma IGF-1 complex protein (T0); with the addition of 12-μg Simmental bull seminal plasma IGF-1 complex protein (T1); and with the addition of 24-μg Simmental bull seminal plasma IGF-1 complex protein (T2). The extended semen was packed in 0.25-mL straws and frozen. Post-thawed semen fertility was evaluated based on the following variables: Sperm motility, viability, intact plasma membrane (IPM), malondialdehyde (MDA) levels, capacitation status, and acrosome reaction. The difference in each variable among the groups was evaluated using analysis of variance, followed by Tukey's honestly significant difference test, at a 95% level of significance. Meanwhile, principal component analysis (PCA) was used to identify the principal component of semen fertility among the seven parameters.

Results: The T1 group showed the highest sperm motility, viability, IPM, and percentage of incapacitated sperm and the lowest MDA levels, percentage of capacitated sperm, and acrosome reaction. PCA revealed that sperm motility had a moderate to very robust correlation with other variables and is the most crucial parameter, accounting for 80.79% of all variables.

Conclusion: The IGF-1 complex in Simmental bull seminal plasma was useful for increasing the fertility of post-thawed Kacang buck semen, and sperm motility was the principal component of semen fertility.

Citing Articles

Combination of nanoparticle green tea extract in tris-egg yolk extender and 39 °c thawing temperatures improve the sperm quality of post-thawed Kacang goat semen.

Mustofa I, Susilowati S, Suprayogi T, Oktanella Y, Purwanto D, Akintunde A Anim Reprod. 2023; 19(4):e20220025.

PMID: 36686855 PMC: 9844672. DOI: 10.1590/1984-3143-AR2022-0025.

Green Tea Extract in the Extender Improved the Post-Thawed Semen Quality and Decreased Amino Acid Mutation of Kacang Buck Sperm.

Susilowati S, Mustofa I, Wurlina W, Hernawati T, Oktanella Y, Soeharsono S Vet Sci. 2022; 9(8).

PMID: 36006318 PMC: 9413626. DOI: 10.3390/vetsci9080403.

α-Tocopherol restores semen quality in rats exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin.

Wurlina W, Mustofa I, Meles D, Safitri E, Susilowati S, Mulyati S Vet World. 2022; 15(2):316-323.

PMID: 35400953 PMC: 8980384. DOI: 10.14202/vetworld.2022.316-323.

The enriched Y-bearing sperm combined with delayed fixed-time artificial insemination for obtaining male Simmental crossbred offspring.

Meles D, Mustofa I, Hariadi M, Wurlina W, Susilowati S, Amaliya A Vet World. 2022; 15(1):102-109.

PMID: 35369602 PMC: 8924384. DOI: 10.14202/vetworld.2022.102-109.

A 150 kDa Protein Derived from Bull Seminal Plasma Extended the Survival Time of Kacang Goat Sperm Stored at 5°C.

Susilowati S, Mustofa I, Wurlina W, Triana I, Utama S, Utomo B Vet Med Int. 2021; 2021:1470209.

PMID: 34840715 PMC: 8616707. DOI: 10.1155/2021/1470209.

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