Europium-labelled Target Cells in an Assay of Natural Killer Cell Activity. I. A Novel Non-radioactive Method Based on Time-resolved Fluorescence

Overview

Journal J Immunol Methods

Publisher Elsevier

Specialties Allergy & Immunology
Pathology

Date 1986 Feb 12

PMID 3456003

Citations 19

Authors

K Blomberg

C Granberg

I Hemmila

T Lovgren

Affiliations

Soon will be listed here.

Abstract

The use of a new marker for labelling cells used as targets for natural killer cells is described. The human erythroleukaemic cell line K-562 was used as target. The cells were labelled with europium diethylenetriaminopentaacetate (EuDTPA) chelates. The detection of the released marker is based on time-resolved fluorometry. The results obtained show that the method is sensitive, specific and rapid. The high specific activity of the marker and the sensitivity of the detection apparatus result in numeric values (counts per second) which are 10-20 times higher than the values (counts per minute) obtained with 51chromium. In comparison with 51chromium release assay the labelling of target cells is less time consuming, the marker release more rapid and the detection time of released marker only 1 second per tube. The use of this non-radioactive marker is an alternative way of measuring natural killer cell mediated cytolysis of target cells.

Citing Articles

Effectiveness of Amplified Natural Killer (ANK) Therapy for Adult T-cell Leukemia/Lymphoma (ATL) and Future Prospects of ANK Therapy.

Nagai K, Nagai S Cancer Med J. 2022; 5(Suppl 5):27-33.

PMID: 35403177 PMC: 8986168.

Cancer immunotherapy with recombinant poliovirus induces IFN-dominant activation of dendritic cells and tumor antigen-specific CTLs.

Brown M, Holl E, Boczkowski D, Dobrikova E, Mosaheb M, Chandramohan V Sci Transl Med. 2017; 9(408).

PMID: 28931654 PMC: 6034685. DOI: 10.1126/scitranslmed.aan4220.

Antibody Functional Assays as Measures of Fc Receptor-Mediated Immunity to HIV - New Technologies and their Impact on the HIV Vaccine Field.

Wines B, Billings H, McLean M, Kent S, Hogarth P Curr HIV Res. 2017; 15(3):202-215.

PMID: 28322167 PMC: 5543561. DOI: 10.2174/1570162X15666170320112247.

Luciferase mRNA Transfection of Antigen Presenting Cells Permits Sensitive Nonradioactive Measurement of Cellular and Humoral Cytotoxicity.

Omokoko T, Luxemburger U, Bardissi S, Simon P, Utsch M, Breitkreuz A J Immunol Res. 2016; 2016:9540975.

PMID: 27057556 PMC: 4736447. DOI: 10.1155/2016/9540975.

Ex vivo generation of dendritic cells from cryopreserved, post-induction chemotherapy, mobilized leukapheresis from pediatric patients with medulloblastoma.

Nair S, Driscoll T, Boczkowski D, Schmittling R, Reynolds R, Johnson L J Neurooncol. 2015; 125(1):65-74.

PMID: 26311248 PMC: 4592836. DOI: 10.1007/s11060-015-1890-2.