Physical Basis for the Determination of Lumen Shape in a Simple Epithelium

Overview

Journal Nat Commun

Specialty Biology

Date 2021 Sep 24

PMID 34556639

Citations 10

Authors

Claudia G Vasquez

Vipul T Vachharajani

Carlos Garzon-Coral

Alexander R Dunn

Affiliations

Soon will be listed here.

Abstract

The formation of a hollow lumen in a formerly solid mass of cells is a key developmental process whose dysregulation leads to diseases of the kidney and other organs. Hydrostatic pressure has been proposed to drive lumen expansion, a view that is supported by experiments in the mouse blastocyst. However, lumens formed in other tissues adopt irregular shapes with cell apical faces that are bowed inward, suggesting that pressure may not be the dominant contributor to lumen shape in all cases. Here we use live-cell imaging to study the physical mechanism of lumen formation in Madin-Darby Canine Kidney cell spheroids, a canonical cell-culture model for lumenogenesis. We find that in this system, lumen shape reflects basic geometrical considerations tied to the establishment of apico-basal polarity. A physical model incorporating both cell geometry and intraluminal pressure can account for our observations as well as cases in which pressure plays a dominant role.

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