Qualitative and Quantitative Assay for Detection of Circulating Autoantibodies Against Human Aortic Antigen

Increased amount of autoantibodies in human sera are the hallmark of autoimmune diseases ( Wang , 2015 ). In case of known antigen, detection of autoantibodies is done using laboratory based methods. However, in most autoimmune diseases, knowledge of self-antigen is still vague. We have developed an ELISA-based quantitative assay to detect the presence of autoantibodies as well as to measure the circulating autoantibodies in the sera of patients suffering from large vessel vasculitis (LVV), an autoimmune disease ( Chakravarti , 2015 ). Using this assay, we detected the increase in anti-aortic antibodies in LVV patient's sera. We have further verified the results by independent biochemical techniques and found the specificity to be > 94% ( Chakravarti , 2015 ). This method can be uniquely modified to suit any autoimmune, in particular organ specific, disease and thus has wider applications in the detection and quantification of autoantibodies.