Soft Agar Colony Formation Assay As a Hallmark of Carcinogenesis

Overview

Journal Bio Protoc

Specialty Biology

Date 2021 Sep 20

PMID 34541100

Citations 26

Authors

Feng Du

Xiaodi Zhao

Daiming Fan

Affiliations

Soon will be listed here.

Abstract

Soft agar colony formation assay is established to estimate the anchorage-independent growth ability of cells. In this assay, a bottom layer of agar with complete media is poured and solidified first, followed by an upper layer containing a specified number of cells suspended in medium-agar mixture. After two weeks of incubation, the number of colonies will be counted, serving as an indicator of malignancy of tumor cells.

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