Acupuncture Therapy Protects PCOS Patients with Diabetes by Regulating MiR-32-3p/PLA2G4A Pathway

Overview

Journal Am J Transl Res

Specialty General Medicine

Date 2021 Sep 20

PMID 34539997

Citations 7

Authors

Jia Wu

Xinghua Chen

Affiliations

Soon will be listed here.

Abstract

Objective: To investigate the potential miRNA-mRNA network co-expressed in polycystic ovary syndrome (PCOS) and diabetes, and explore the molecular mechanism of traditional acupuncture treatment of PCOS.

Methods: Patients with PCOS and diabetes who had undergone acupuncture treatment from January 2019 to June 2020 were recruited in this study. The potential miRNA-mRNA network co-expressed in PCOS and diabetes was obtained through bioinformatics analysis. The expression levels of candidate gen es were determined using quantitative qRT-PCR to study the effectiveness of acupuncture approach. Further, the mechanism of action of acupuncture method was determined using luciferase assay.

Results: A total of 44 patients were included in this study. The miRNA-mRNA network for PCOS was then constructed based on the results of the bioinformatics analysis. Acupuncture treatment could significantly down-regulate miR-32-3p levels and up-regulate expression of PLA2G4A. Luciferase experiments showed that miR-32-3p could affect glucose metabolism in PCOS patients through down-regulating PLA2G4A expression. Functional and pathway enrichment analysis further suported this finding.

Conclusions: MiR-32-3p regulates PLA2G4A protein expression, which is vital in the pathogenesis of PCOS and diabetes. Further, this research proved that the potential mechanism of traditional acupuncture treatment may be the downregulation of miR-32-3p, thus inhibiting PCOS and diabetes progression.

Citing Articles

MicroRNAs as Biomarkers for Metabolic Disorders in Polycystic Ovary Syndrome (PCOS): A Review.

Dymanowska I, Frankowska K, Krawczyk A, Kociuba J, Gil-Kulik P, Banaszewska B Med Sci Monit. 2024; 30:e946480.

PMID: 39709519 PMC: 11670786. DOI: 10.12659/MSM.946480.

The effects of acupuncture on patients with premature ovarian insufficiency and polycystic ovary syndrome: an umbrella review of systematic reviews and meta-analyses.

Bai T, Deng X, Bi J, Ni L, Li Z, Zhuo X Front Med (Lausanne). 2024; 11:1471243.

PMID: 39655237 PMC: 11627218. DOI: 10.3389/fmed.2024.1471243.

Effects and mechanisms of acupuncture on women related health.

Qin H, Feng J, Wu X Front Med. 2023; 18(1):46-67.

PMID: 38151668 DOI: 10.1007/s11684-023-1051-5.

A Review on Acupuncture Efficiency in Human Polycystic Ovary/Ovarian Syndrome.

de Oliveira N, Machado J, Lopes L, Criado M J Pharmacopuncture. 2023; 26(2):105-123.

PMID: 37405111 PMC: 10315877. DOI: 10.3831/KPI.2023.26.2.105.

Effects of TCM on polycystic ovary syndrome and its cellular endocrine mechanism.

Chen H, Deng C, Meng Z, Meng S Front Endocrinol (Lausanne). 2023; 14:956772.

PMID: 37260441 PMC: 10228207. DOI: 10.3389/fendo.2023.956772.

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