» Articles » PMID: 34539990

MiR-590-5p Affects Chondrocyte Proliferation, Apoptosis, and Inflammation by Targeting FGF18 in Osteoarthritis

Overview
Journal Am J Transl Res
Specialty General Medicine
Date 2021 Sep 20
PMID 34539990
Citations 7
Authors
Affiliations
Soon will be listed here.
Abstract

Objective: To investigate the potential miRNA targeting FGF18, and its role in regulating the proliferation, apoptosis and inflammation in human primary chondrocytes.

Methods: The normal human chondrocytes were induced by IL-1β to mimic OA . qPCR and Western blotting were performed to evaluate the expression of FGF18. Target Scan analysis was performed to predict the miRNA targeting FGF18. Then, the expression of miR-590-5p was quantified by qPCR in IL-1β-induced chondrocytes. After transfection of miR-590-5p mimics or inhibitors, CCK-8 assay was conducted to determine the cell viability and apoptosis-related proteins, and cartilage degeneration related biomarkers were assayed by qPCR and Western blotting. The levels of tumor necrosis factor (TNF)-α, interleukin (IL)-6 and IL-8 were determined by ELISA. The targeting relationship between miR-590-5p and FGF18 was assayed by luciferase reporter assay in IL-1β-induced chondrocytes.

Results: Target Scan analysis predicted that FGF18 is directly targeted by miR-590-5p. miR-590-5p was up-regulated, whereas FGF18 expression was inhibited in IL-1β-induced chondrocytes. miR-590-5p mimics reduced the expression of FGF18 protein, inhibited the cell viability of chondrocytes, and promoted secretion of inflammatory factors in chondrocytes, while miR-590-5p inhibitors increased FGF18 levels in IL-1β-treated chondrocytes. Furthermore, expression of inflammatory factors in chondrocytes was reduced by miR-590-5p inhibitors. The luciferase reporter assay showed that miR-590-5p could target FGF18.

Conclusions: miR-590-5p promotes OA progression by targeting FGF18, which serves as a potential therapeutic target for OA.

Citing Articles

Serum and Plasma miRNA Expression Levels in Sudden Sensorineural Hearing Loss.

Nunez D, Abgoon R, Wijesinghe P, Garnis C Int J Mol Sci. 2025; 26(3).

PMID: 39941013 PMC: 11818497. DOI: 10.3390/ijms26031245.


The potential molecular markers of inflammatory response in KOA with AD based on single-cell transcriptome sequencing analysis and identification of ligands by virtual screening.

Wu Y, Chen W, Jian J, Liu W, Wang H, Gao D Mol Divers. 2024; 29(1):319-336.

PMID: 38622351 DOI: 10.1007/s11030-024-10854-4.


Analysis of miRNAs in Caused by Mutations in and : Insights into Molecular Mechanisms and Potential Therapeutic Targets.

Botor M, Augusciak-Duma A, Lesiak M, Sieron L, Dziedzic-Kowalska A, Witecka J Pharmaceuticals (Basel). 2023; 16(10).

PMID: 37895885 PMC: 10609877. DOI: 10.3390/ph16101414.


FGF18 alleviates hepatic ischemia-reperfusion injury via the USP16-mediated KEAP1/Nrf2 signaling pathway in male mice.

Tong G, Chen Y, Chen X, Fan J, Zhu K, Hu Z Nat Commun. 2023; 14(1):6107.

PMID: 37777507 PMC: 10542385. DOI: 10.1038/s41467-023-41800-x.


Downregulation of lncRNA NEAT1 interacts with miR-374b-5p/PGAP1 axis to aggravate the development of osteoarthritis.

Huang F, Su Z, Yang J, Zhao X, Xu Y J Orthop Surg Res. 2023; 18(1):670.

PMID: 37691099 PMC: 10494329. DOI: 10.1186/s13018-023-04147-z.


References
1.
Zhang J, Zhou Y, Huang T, Wu F, Pan Y, Dong Y . FGF18, a prominent player in FGF signaling, promotes gastric tumorigenesis through autocrine manner and is negatively regulated by miR-590-5p. Oncogene. 2018; 38(1):33-46. PMC: 6318220. DOI: 10.1038/s41388-018-0430-x. View

2.
Li Y, Yuan F, Song Y, Guan X . miR-17-5p and miR-19b-3p prevent osteoarthritis progression by targeting EZH2. Exp Ther Med. 2020; 20(2):1653-1663. PMC: 7388554. DOI: 10.3892/etm.2020.8887. View

3.
Ding Y, Wang L, Zhao Q, Wu Z, Kong L . MicroRNA‑93 inhibits chondrocyte apoptosis and inflammation in osteoarthritis by targeting the TLR4/NF‑κB signaling pathway. Int J Mol Med. 2018; 43(2):779-790. PMC: 6317687. DOI: 10.3892/ijmm.2018.4033. View

4.
Wang X, Zhao F, Yi L, Tang J, Zhu Z, Pang Y . MicroRNA-21-5p as a novel therapeutic target for osteoarthritis. Rheumatology (Oxford). 2019; . DOI: 10.1093/rheumatology/kez102. View

5.
Lieben L . Osteoarthritis: Osteophyte formation involves PAR2. Nat Rev Rheumatol. 2016; 12(2):70-1. DOI: 10.1038/nrrheum.2016.6. View