Comparative Inter- and IntraSpecies Transcriptomics Revealed Key Differential Pathways Associated With Aluminium Stress Tolerance in Lentil

Overview

Journal Front Plant Sci

Date 2021 Sep 17

PMID 34531881

Authors

Chandan Kumar Singh

Dharmendra Singh

Jyoti Taunk

Priya Chaudhary

Ram Sewak Singh Tomar

Shivani Chandra

Deepti Singh

Madan Pal

Noren Singh Konjengbam

M Premjit Singh

Rakesh Singh Sengar

Ashutosh Sarker

Affiliations

Soon will be listed here.

Abstract

Aluminium stress causes plant growth retardation and engenders productivity loss under acidic soil conditions. This study accentuates morpho-physiological and molecular bases of aluminium (Al) tolerance within and between wild (ILWL-15) and cultivated (L-4602 and BM-4) lentil species. Morpho-physiological studies revealed better cyto-morphology of tolerant genotypes over sensitive under Al stress conditions. Mitotic lesions were observed in root cells under these conditions. Transcriptome analysis under Al stress revealed 30,158 specifically up-regulated genes in different comparison groups showing contigs between 15,305 and 18,861 bp. In tolerant genotypes, top up-regulated differentially expressed genes (DEGs) were found to be involved in organic acid synthesis and exudation, production of antioxidants, callose synthesis, protein degradation, and phytohormone- and calcium-mediated signalling under stress conditions. DEGs associated with epigenetic regulation and Al sequestration inside vacuole were specifically upregulated in wild and cultivars, respectively. Based on assembled unigenes, an average of 6,645.7 simple sequence repeats (SSRs) and 14,953.7 high-quality single nucleotide polymorphisms (SNPs) were spotted. By quantitative real-time polymerase chain reaction (qRT-PCR), 12 selected genes were validated. Gene ontology (GO) annotation revealed a total of 8,757 GO terms in three categories, ., molecular, biological, and cellular processes. Kyoto Encyclopaedia of Genes and Genomes pathway scanning also revealed another probable pathway pertaining to metacaspase-1,-4, and -9 for programmed cell death under Al-stress conditions. This investigation reveals key inter- and intraspecies metabolic pathways associated with Al-stress tolerance in lentil species that can be utilised in designing future breeding programmes to improve lentil and related species towards Al stress.

Citing Articles

Genome wide identification of MATE and ALMT gene family in lentil (Lens culinaris Medikus) and expression profiling under Al stress condition.

Tripathi A, Singh D, Bhati J, Singh D, Taunk J, Alkahtani J BMC Plant Biol. 2025; 25(1):88.

PMID: 39844062 PMC: 11753098. DOI: 10.1186/s12870-025-06086-9.

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