Upregulated CircRAD18 Promotes Tumor Progression by Reprogramming Glucose Metabolism in Papillary Thyroid Cancer

Overview

Journal Gland Surg

Publisher AME Publishing Company

Specialty Endocrinology

Date 2021 Sep 16

PMID 34527562

Citations 10

Authors

Wenkuan Chen

Tingting Zhang

Yanfang Bai

Hong Deng

Fang Yang

Renjie Zhu

Yingle Chen

Zheng He

Qi Zeng

Ming Song

Affiliations

Soon will be listed here.

Abstract

Background: By regulating complex functional processes, circRNAs are crucial in the development of different cancers. Nevertheless, most circRNAs in papillary thyroid cancer metabolic reprogramming remain unknown.

Methods: The expression of circRNA was assessed by qRT-PCR in papillary thyroid cancer tissues and cell lines. Cell proliferation and glucose intake experiments were performed by certain kit. Transwell assays and wound healing assays were performed to investigate the function of circRNA in metastasis. In addition, a serious of molecular experiments were conducted to determine the exact mechanism of circRAD18. Luciferase reporter and RNA immunoprecipitation assay were conducted to determine the molecular interaction between circRNA and miRNA.

Results: We characterized circRAD18 as a significantly upregulated circRNA in papillary thyroid tissues and cell lines and found its downregulation could inhibit the growth and metastasis ability of papillary thyroid cancer. Interestingly, we found that circRAD18 was involved in glucose metabolism reprogramming of papillary thyroid cancer, and its silence could remarkably inhibit cell glucose uptake and lactate production in papillary thyroid cancer cells. Inhibition of circRAD18 could decrease the expression level of PDK1 protein by sponging miR-516b.

Conclusions: This study verified the novel function of the circRAD18-miR-516b-PDK1 axis in papillary thyroid cancer metabolic reprogramming progression, which has potential to be a novel therapeutic target.

Citing Articles

Reprogramming of Thyroid Cancer Metabolism: from Mechanism to Therapeutic Strategy.

Wan Y, Li G, Cui G, Duan S, Chang S Mol Cancer. 2025; 24(1):74.

PMID: 40069775 PMC: 11895238. DOI: 10.1186/s12943-025-02263-4.

Circular RNAs in cancer.

Guo Y, Huang Q, Heng Y, Zhou Y, Chen H, Xu C MedComm (2020). 2025; 6(2):e70079.

PMID: 39901896 PMC: 11788016. DOI: 10.1002/mco2.70079.

hsa_circ_0101050 regulated by ZC3H13 enhances tumorigenesis in papillary thyroid cancer via mA modification.

Lv K, Xie P, Yang Q, Luo M, Li C Heliyon. 2024; 10(12):e32913.

PMID: 38988519 PMC: 11234023. DOI: 10.1016/j.heliyon.2024.e32913.

The application of plasma circRAD18 in the prediction of gestational diabetes mellitus (GDM) and its adverse effects.

Ni N, Li L, Xiao M, Yu F BMC Pregnancy Childbirth. 2024; 24(1):308.

PMID: 38658867 PMC: 11041028. DOI: 10.1186/s12884-024-06302-8.

The emerging role and clinical significance of circRNAs in papillary thyroid cancer.

Ma J, Xu J, Zhang X, Quan J Front Endocrinol (Lausanne). 2024; 15:1351776.

PMID: 38544689 PMC: 10965563. DOI: 10.3389/fendo.2024.1351776.

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