A Cooperative DNA Catalyst

Overview

Journal J Am Chem Soc

Publisher American Chemical Society

Specialty Chemistry

Date 2021 Sep 15

PMID 34524797

Citations 3

Authors

Dallas N Taylor

Samuel R Davidson

Lulu Qian

Affiliations

Soon will be listed here.

Abstract

DNA catalysts are fundamental building blocks for diverse molecular information-processing circuits. Allosteric control of DNA catalysts has been developed to activate desired catalytic pathways at desired times. Here we introduce a new type of DNA catalyst that we call a cooperative catalyst: a pair of reversible reactions are employed to drive a catalytic cycle in which two signal species, which can be interpreted as an activator and an input, both exhibit catalytic behavior for output production. We demonstrate the role of a dissociation toehold in controlling the kinetics of the reaction pathway and the significance of a wobble base pair in promoting the robustness of the activator. We show near-complete output production with input and activator concentrations that are 0.1 times the gate concentration. The system involves just a double-stranded gate species and a single-stranded fuel species, as simple as the seesaw DNA catalyst, which has no allosteric control. The simplicity and modularity of the design make the cooperative DNA catalyst an exciting addition to strand-displacement motifs for general-purpose computation and dynamics.

Citing Articles

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Leveraging Steric Moieties for Kinetic Control of DNA Strand Displacement Reactions.

Lysne D, Hachigian T, Thachuk C, Lee J, Graugnard E J Am Chem Soc. 2023; 145(30):16691-16703.

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