[Drug-resistant Gene Polymorphisms in Isolated from Bioko Island, Equatorial Guinea in 2018 and 2019]

Objective: To investigate the genetic polymorphisms of multidrug resistance protein 1 (), chloroquine resistance transporter () and () genes in Bioko Island, Equatorial Guinea, so as to provide insights into the development of the malaria control strategy in local areas.

Methods: A total of 85 peripheral blood samples were collected from patients with infections in Bioko Island, Equatorial Guinea in 2018 and 2019, and genomic DNA was extracted. The , and genes were amplified using a nested PCR assay. The amplification products were sequenced, and the gene sequences were aligned.

Results: There were no mutations associated with artemisinin resistance in gene in Bioko Island, Equatorial Guinea, while drug-resistant mutations were detected in and genes, and the proportions of PfMDR1_N86Y, PfMDR1_Y184F and PfCRT_K76T mutations were 35.29% (30/85), 72.94% (62/85) and 24.71% (21/85), respectively.

Conclusions: There are mutations in , and genes in isolates from Bioko Island, Equatorial Guinea.