R2R3-MYB Transcription Factor SmMYB52 Positively Regulates Biosynthesis of Salvianolic Acid B and Inhibits Root Growth in

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2021 Sep 10

PMID 34502445

Citations 10

Authors

Rao Yang

Shengsong Wang

Haolan Zou

Lin Li

Yonghui Li

Donghao Wang

Hongxing Xu

Xiaoyan Cao

Affiliations

Soon will be listed here.

Abstract

The dried root of is a renowned traditional Chinese medicine that was used for over 1000 years in China. Salvianolic acid B (SalB) is the main natural bioactive product of . Although many publications described the regulation mechanism of SalB biosynthesis, few reports simultaneously focused on root development. For this study, an R2R3-MYB transcription factor gene () was overexpressed and silenced, respectively, in sterile seedlings. We found that significantly inhibited root growth and indole-3-acetic acid (IAA) accumulation, whereas it activated phenolic acid biosynthesis and the jasmonate acid (JA) signaling pathway. Quantitative real-time polymerase chain reaction (qRT-PCR) analyses revealed that MYB52 suppressed the transcription levels of key enzyme-encoding genes involved in the IAA biosynthetic pathway and activated key enzyme-encoding genes involved in the JA and phenolic acid biosynthesis pathways. In addition, yeast one-hybrid (Y1H) and dual-luciferase assay showed that MYB52 directly binds to and activates the promoters of several key enzyme genes for SalB biosynthesis, including , , , and , to promote the accumulation of SalB. This is the first report of a regulator that simultaneously affects root growth and the production of phenolic acids in .

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